Functionalized 3D-Printed ST2/Gelatin Methacryloyl/Polcaprolactone Scaffolds for Enhancing Bone Regeneration with Vascularization

作者全名:"Liu, Guangliang; Chen, Jie; Wang, Xiaofang; Liu, Yujiao; Ma, Yufei; Tu, Xiaolin"

作者地址:"[Liu, Guangliang; Chen, Jie; Wang, Xiaofang; Liu, Yujiao; Ma, Yufei; Tu, Xiaolin] Chongqing Med Univ, Inst Life Sci, Lab Skeletal Dev & Regenerat, Chongqing 400016, Peoples R China"

通信作者:"Ma, YF; Tu, XL (通讯作者),Chongqing Med Univ, Inst Life Sci, Lab Skeletal Dev & Regenerat, Chongqing 400016, Peoples R China."

来源:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ESI学科分类:CHEMISTRY

WOS号:WOS:000838836000001

JCR分区:Q2

影响因子:5.6

年份:2022

卷号:23

期号:15

开始页: 

结束页: 

文献类型:Article

关键词:3D-printing; Wnt3a; scaffold; bone regeneration; vascularization

摘要:"Growth factors were often used to improve the bioactivity of biomaterials in order to fabricate biofunctionalized bone grafts for bone defect repair. However, supraphysiological concentrations of growth factors for improving bioactivity could lead to serious side effects, such as ectopic bone formation, radiculitis, swelling of soft tissue in the neck, etc. Therefore, safely and effectively applying growth factors in bone repair biomaterials comes to be an urgent problem that needs to be addressed. In this study, an appropriate concentration (50 ng/mL) of Wnt3a was used to pretreat the 3D-bioprinting gelatin methacryloyl(GelMA)/polycaprolactone(PCL) scaffold loaded with bone marrow stromal cell line ST2 for 24 h. This pretreatment promoted the cell proliferation, osteogenic differentiation, and mineralization of ST2 in the scaffold in vitro, and enhanced angiogenesis and osteogenesis after being implanted in critical-sized mouse calvarial defects. On the contrary, the inhibition of Wnt/beta-catenin signaling in ST2 cells reduced the bone repair effect of this scaffold. These results suggested that ST2/GelMA/PCL scaffolds pretreated with an appropriate concentration of Wnt3a in culture medium could effectively enhance the osteogenic and angiogenic activity of bone repair biomaterials both in vitro and in vivo. Moreover, it would avoid the side effects caused by the supraphysiological concentrations of growth factors. This functionalized scaffold with osteogenic and angiogenic activity might be used as an outstanding bone substitute for bone regeneration and repair."

基金机构:"National Natural Science Foundation of China [U1601220, 81672118, 82072450, 82002310]; CQMU Program for Youth Innovation in Future Medicine [W0075]; Chongqing Postgraduate Research and Innovation Project [CYB20167]"

基金资助正文:"This research was funded by the National Natural Science Foundation of China U1601220 (X.T.), 81672118 (X.T.), 82072450 (X.T.), 82002310 (Y.M.), CQMU Program for Youth Innovation in Future Medicine, W0075 (Y.M.), and the Chongqing Postgraduate Research and Innovation Project CYB20167 (J.C.)."