Effects of phytosterols' intake on systemic and tissue-specific lipid metabolism in C57BL/6J mice

作者全名："Zhu, Qian; Wu, Jingjing; Li, Jianling; Wang, Shengquan; He, Daxue; Lian, Xuemei"

作者地址："[Zhu, Qian; Wu, Jingjing; Lian, Xuemei] Chongqing Med Univ, Ctr Lipid Res, Key Lab Mol Biol Infect Dis, Minist Educ, Chongqing, Peoples R China; [Zhu, Qian; Li, Jianling; Wang, Shengquan; He, Daxue; Lian, Xuemei] Chongqing Med Univ, Sch Publ Hlth & Management, Dept Nutr & Food Hyg, Chongqing, Peoples R China; [Wu, Jingjing] Qiannan Ctr Dis Control & Prevent, Duyun, Peoples R China"

通信作者："Lian, XM (通讯作者)，Chongqing Med Univ, Ctr Lipid Res, Key Lab Mol Biol Infect Dis, Minist Educ, Chongqing, Peoples R China.; Lian, XM (通讯作者)，Chongqing Med Univ, Sch Publ Hlth & Management, Dept Nutr & Food Hyg, Chongqing, Peoples R China."

来源：FRONTIERS IN NUTRITION

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000839032300001

JCR分区：Q2

影响因子：5

年份：2022

卷号：9

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：phytosterols; cholesterol; lipid metabolism; tissue specificity; RNA-seq

摘要："This study aimed to investigate the long-term effects of phytosterols (PS) intake on systemic and tissue-specific lipid metabolism in C57BL/6J mice. Healthy male C57BL/6J mice were randomly divided into control diet group (CS) and PS diet group (2% PS). After 28 weeks of continuous feeding, serums, livers, and lungs were collected for targeted free sterols quantification, biochemical tests, lipid profile detection, and RNA-seq analysis. Compared with the CS group, 2% PS supplementation significantly increased campesterol concentrations and its ratio to cholesterol in the serum, liver, and lung of mice, with cholestanol concentrations and its ratio to cholesterol decreased. Total cholesterol (TC) levels were reduced in the serum of the PS group (p < 0.05), with the triglyceride (TG) levels unchanged. In response to the decreased circulating cholesterol concentration, the expression of endogenous cholesterol synthesis genes was upregulated in the liver, but caused no obvious lipid accumulation and inflammatory cell infiltration. However, for peripheral tissues, long-term PS-fed mice exhibited diminished cholesterol synthesis, fatty acid transport, and oxidation in the lung. The results provided clear indication that 2% PS diet effectively reduced circulating TC levels in the healthy mice, with tissue-specific lipid metabolic regulation in the liver and the lung."

基金机构："Yihaikerry Nutrition and Food Safety Foundation, Chinese Nutrition Society [CNS-W2018A45]; project of Technology Innovation and Application, Chongqing, China [cstc2019jscx-msxmX0280]; project of the top-notch talent cultivation program for the graduate students of Chongqing Medical University [BJRC202029]"

基金资助正文："The study was funded by the Yihaikerry Nutrition and Food Safety Foundation, Chinese Nutrition Society (CNS-W2018A45); the project of Technology Innovation and Application, Chongqing, China (cstc2019jscx-msxmX0280); and the project of the top-notch talent cultivation program for the graduate students of Chongqing Medical University (BJRC202029)."