Identification of G6PC as a potential prognostic biomarker in hepatocellular carcinoma based on bioinformatics analysis

作者全名："Tian, Li; Liao, Yong"

作者地址："[Tian, Li; Liao, Yong] Minist Educ, Key Lab Mol Biol Infect Dis, Chongqing, Peoples R China; [Tian, Li; Liao, Yong] Chongqing Med Univ, Inst Viral Hepatitis, Chongqing, Peoples R China; [Tian, Li; Liao, Yong] Chongqing Med Univ, Affiliated Hosp 2, Dept Infect Dis, Chongqing, Peoples R China"

通信作者："Liao, Y (通讯作者)，Minist Educ, Key Lab Mol Biol Infect Dis, Chongqing, Peoples R China.; Liao, Y (通讯作者)，Chongqing Med Univ, Inst Viral Hepatitis, Chongqing, Peoples R China.; Liao, Y (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Infect Dis, Chongqing, Peoples R China."

来源：MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000841935100022

JCR分区：Q2

影响因子：1.6

年份：2022

卷号：101

期号：33

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结束页：　

文献类型：Article

关键词：bioinformatics; differentially expressed genes; glucose-6-phosphatase catalytic; hepatocellular carcinoma; prognosis

摘要："Hepatocellular carcinoma (HCC) has high mortality and incidence rates around the world with limited therapeutic options. There is an urgent need for identification of novel therapeutic targets and biomarkers for early diagnosis and predicting patient survival with HCC. Several studies (GSE102083, GSE29722, GSE101685, and GSE112790) from the GEO database in HCC were screened and analyzed by GEO2R, gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were conducted with the Database for Annotation, Visualization and Integrated Discovery. The protein-protein interaction network was plotted and the module analysis was performed using Search Tool for the Retrieval of Inter-acting Genes/Proteins database and Cytoscape. The expression and survival of key genes were identified using UALCAN, Kaplan-Meier Plotter and ONCOMINE online databases, and the immune infiltration level of key genes was analyzed via the Tumor Immune Estimation Resource (TIMER) database. Through database analysis, eight key genes were finally screened out, and the expressions of cyclin-dependent kinase regulatory subunit 2 and glucose-6-phosphatase catalytic (G6PC), which were closely related to the survival of HCC patients, was detected by using UALCAN. Further analysis on the differential expression of G6PC in multiple cancerous tumors and normal tissues revealed low expression in many solid tumors by Oncomine and TIMER. In addition, Kaplan-Meier plotter and UALCAN database analysis to access diseases prognosis suggested that low expression of G6PC was significantly associated with poor overall survival in HCC patients. Finally, TIMER database analysis showed a significant negative correlation between G6PC and infiltration levels of six kinds of immune cells. The somatic copy number alterations of G6PC were associated with B cells, CD8(+) T cells, CD4(+) T cells, macrophages, dentritic cells and neutrophils. These bioinformatic data identified G6PC as a potential key gene in the diagnosis and prognosis of HCC."

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