Hypotonic induction of aquaporin5 expression in rat astrocytes through p38 MAPK pathway

作者全名："Yi, Yaoxing; Qiu, Guoping; Liu, Hui; Gao, Fei; Liu, Xueyuan; Chen, Yuqing; Yang, Mei"

作者地址："[Yi, Yaoxing; Qiu, Guoping; Liu, Hui; Liu, Xueyuan; Chen, Yuqing; Yang, Mei] Chongqing Med Univ, Coll Basic Med, Inst Neurosci, Dept Anat, Chongqing 400016, Peoples R China; [Yi, Yaoxing] Chongqing Med Univ, Lab Teaching, Chongqing, Peoples R China; [Yi, Yaoxing] Chongqing Med Univ, Management Ctr, Chongqing, Peoples R China; [Gao, Fei] Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, Chongqing, Peoples R China"

通信作者："Yang, M (通讯作者)，Chongqing Med Univ, Coll Basic Med, Inst Neurosci, Dept Anat, Chongqing 400016, Peoples R China."

来源：ANATOMIA HISTOLOGIA EMBRYOLOGIA

ESI学科分类：PLANT & ANIMAL SCIENCE

WOS号：WOS:000844247100001

JCR分区：Q3

影响因子：0.9

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：aquaporin; astrocyte; brain oedema; hypotension; MAPK

摘要："Brain oedema is a common pathological phenomenon following many diseases and may lead to severe secondary damage. Astrocytes are the most numerous cells in the brain. Five aquaporins (AQPs) have been found in mature astrocytes, which play crucial roles in water transportation. However, most studies have focused on AQP4 or AQP9 and whether another aquaporin such as AQP5 involved in brain oedema is unclear. Here, we addressed the issue that the expression pattern of AQP5 in rat astrocytes in vitro was altered in the hypotonic condition through some mitogen-activated protein kinases (MAPK) pathways. Primary astrocytes were randomly divided into the control group and the hypotonic group. Cell viability was evaluated by MTT test. Immunofluorescence, Western blotting and real-time PCR were used to detect the expression of AQP5. Western blotting was used to detect the variation of MAPK pathway. The present study demonstrated that incubation of astrocytes in the hypotonic medium produced an increase inAQP5 expression, and AQP5 peaked at 6-12 h after hypotension solution exposure. In addition, MAPK pathways were set in motion under hypotension, but not all branches. Only the p38 inhibitor can inhibit AQP5 expression in cultured astrocytes. AQP5 is directly related to the extracellular hypotonic stimuli in astrocytes, which could be regulated through the p38 MAPK pathway."

基金机构："National Natural Science Foundation of China [81971230, 81671312, 81672893]; Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJQN201800428]; Foundation and Cutting-Edge Research Plan of Chongqing [cstc2017jcyjAX0414]; Chongqing postgraduate research and innovation project [CYS16118]"

基金资助正文："The study was supported by the National Natural Science Foundation of China (81971230 and 81671312), Scientific and Technological Research Program of Chongqing Municipal Education Commission (KJQN201800428) to Mei Yang, National Natural Science Foundation of China (81672893) to Fei Gao, Foundation and Cutting-Edge Research Plan of Chongqing (cstc2017jcyjAX0414) to Guoping Qiu, Chongqing postgraduate research and innovation project (CYS16118) to Yaoxing Yi."