Engineering the Surface Properties of DNA Nanostructures by Tuning the Valency of Assembling Species for Biomedical Applications

作者全名："Liu, Qian; Xia, Jingruo; Yu, Qingtao; Gu, Pengcheng; Yuan, Yue; Liu, Ke; Huang, Chaowang; Chen, Chunfa; Guo, Xiaolan; Qian, Hang"

作者地址："[Liu, Qian; Yu, Qingtao; Gu, Pengcheng; Liu, Ke] Chongqing Med Univ, Lab Pharm & Chem, Lab Teaching & Management Ctr, Chongqing 400016, Peoples R China; [Liu, Qian; Yu, Qingtao; Gu, Pengcheng; Liu, Ke] Chongqing Med Univ, Lab Tissue & Cell Biol, Lab Teaching & Management Ctr, Chongqing 400016, Peoples R China; [Xia, Jingruo; Guo, Xiaolan] North Sichuan Med Univ, Affiliated Hosp, Dept Lab Med, Nanchong 637000, Sichuan, Peoples R China; [Yuan, Yue; Huang, Chaowang; Chen, Chunfa; Qian, Hang] Third Mil Med Univ, Xinqiao Hosp, Inst Resp Dis, 183 Xinqiao St, Chongqing 400037, Peoples R China"

通信作者："Liu, Q (通讯作者)，Chongqing Med Univ, Lab Pharm & Chem, Lab Teaching & Management Ctr, Chongqing 400016, Peoples R China.; Liu, Q (通讯作者)，Chongqing Med Univ, Lab Tissue & Cell Biol, Lab Teaching & Management Ctr, Chongqing 400016, Peoples R China.; Guo, XL (通讯作者)，North Sichuan Med Univ, Affiliated Hosp, Dept Lab Med, Nanchong 637000, Sichuan, Peoples R China.; Qian, H (通讯作者)，Third Mil Med Univ, Xinqiao Hosp, Inst Resp Dis, 183 Xinqiao St, Chongqing 400037, Peoples R China."

来源：MACROMOLECULAR BIOSCIENCE

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000848852100001

JCR分区：Q2

影响因子：4.6

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：DNA nanostructures; magnesium-free assembly; noncanonical assembly; polyamines

摘要："Self-assembled DNA nanostructures hold great potentials in biomedical applications. Nevertheless, the negatively charged DNA backbone and susceptivity to enzyme degradation pose challenges to this regard. Engineering the surface properties of DNA nanostructures by assembling DNA with guest molecules in magnesium-free system is promising to solve these issues. In this study, the polyamines-mediated DNA self-assembly with an emphasis on the valency of polyamines is investigated. Both spermine, spermidine, and putrescine can assemble DNA tetrahedron under appropriate concentrations. The cytotoxicity and cellular uptake efficiencies vary with the polyamine valency. Compared with magnesium-assembled DNA tetrahedron, polyamine-assembled DNA tetrahedron exhibits higher cellular uptake efficiency and serum stability. Circular dichroism spectrum results indicate that polyamines induce DNA conformation slightly shifting from B form to A form. The improved performances of polyamine-assembled DNA tetrahedrons under physiological settings are attributed to the surface properties that altered by guest molecules polyamine. The current study suggests that engineering the surface properties of DNA nanostructures by assembling them with guest cationic species is promising to further their biomedical applications."

基金机构："National Natural Science Foundation of China [32071379]; Natural Science Foundation of Chongqing, China [cstc2020jcyjmsxmX0622]; Scientific and Technological Research Program of Chongqing Municipal Eduacation Commission [KJQN201900405]; Project of Tutorial System of Medical Undergraduate in Lab Teaching & Management Center in Chongqing Medical University [LTMCMTS201907]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (32071379), the Natural Science Foundation of Chongqing, China (No. cstc2020jcyjmsxmX0622), the Project Supported by Scientific and Technological Research Program of Chongqing Municipal Eduacation Commission (KJQN201900405), and the Project of Tutorial System of Medical Undergraduate in Lab Teaching & Management Center in Chongqing Medical University LTMCMTS201907."