Elevated intraspinal pressure drives edema progression after acute compression spinal cord injury in rabbits

作者全名："Yang, Chaohua; He, Tao; Wang, Qing; Wang, Gaoju; Ma, Jingjin; Chen, Zhiyu; Li, Qiaochu; Wang, Linbang; Quan, Zhengxue"

作者地址："[Yang, Chaohua; He, Tao; Ma, Jingjin; Chen, Zhiyu; Li, Qiaochu; Wang, Linbang; Quan, Zhengxue] Chongqing Med Univ, Dept Orthoped Surg, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Yang, Chaohua; Wang, Qing; Wang, Gaoju] Southwest Med Univ, Dept Orthopaed, Affiliated Hosp, 25 Taiping St, Luzhou 646000, Sichuan, Peoples R China; [Yang, Chaohua; He, Tao; Ma, Jingjin; Chen, Zhiyu; Li, Qiaochu; Wang, Linbang; Quan, Zhengxue] Chongqing Med Univ, Orthoped Lab, 1 Youyi Rd, Chongqing 400016, Peoples R China; [He, Tao] Chongqing Gen Hosp, Dept Orthopaed Trauma, 118 Xingguang Ave, Chongqing 40114, Peoples R China"

通信作者："Quan, ZX (通讯作者)，Chongqing Med Univ, Dept Orthoped Surg, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：EXPERIMENTAL NEUROLOGY

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000850882200004

JCR分区：Q1

影响因子：5.3

年份：2022

卷号：357

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Intraspinal pressure; Edema; Spinal cord injury; Diffusion tensor tractography; Arterial spin labeling; Blood spinal cord barrier

摘要："Elevated intraspinal pressure (ISP) following traumatic spinal cord injury (tSCI) can be an important factor for secondary SCI that may result in greater tissue damage and functional deficits. Our present study aimed to investigate the dynamic changes in ISP after different degrees of acute compression SCI in rabbits with closed canals and explore its influence on spinal cord pathophysiology. Closed balloon compression injuries were induced with different inflated volumes (40 mu l, 50 mu l or no inflation) at the T7/8 level in rabbits. ISP was monitored by a SOPHYSA probe at the epicenter within 7 days post-SCI. Edema progression, spinal cord perfusion and damage severity were evaluated by serial multisequence MRI scans, somatosensory evoked po-tentials (SEPs) and behavioral scores. Histological and blood spinal cord barrier (BSCB) permeability results were subsequently analyzed. The results showed that the ISP waveforms comprised three peaks, significantly increased after tSCI, peaked at 72 h (21.86 +/- 3.13 mmHg) in the moderate group or 48 h (31.71 +/- 6.02 mmHg) in the severe group and exhibited ""slow elevated and fast decreased "" or ""fast elevated and slow decreased "" dynamic changes in both injured groups. Elevated ISP after injury was correlated with spinal cord perfusion and edema progression, leading to secondary lesion enlargement. The secondary damage aggravation can be visualized by diffusion tensor tractography (DTT). Moreover, the BSCB permeability was significantly increased at the epicenter and rostrocaudal segments at 72 h after SCI; by 14 days, notable permeability was still observed at the caudal segment in the severely injured rabbits. Our results suggest that the ISP of rabbits with closed canals increased after acute compression SCI and exhibited different dynamic change patterns in moderately and severely injured rabbits. Elevated ISP exacerbated spinal cord perfusion, drove edema progression and led to secondary lesion enlargement that was strongly associated with BSCB disruption. For severe tSCI, early inter-vention targeting elevated ISP may be an indispensable choice to rescue spinal cord function."

基金机构：Chongqing Yingcai Plan [cstc2021ycjh-bgzxm0041]; Southwest Medical University [2018-ZRQN-110]

基金资助正文："Funding This work was funded by the Chongqing Yingcai Plan (cstc2021ycjh-bgzxm0041) , and the Southwest Medical University (2018-ZRQN-110) ."