Revealing the Therapeutic Targets and Mechanism of Ginsenoside Rg1 for Liver Damage Related to Anti-Oxidative Stress Using Proteomic Analysis

作者全名："Hou, Jiying; Ma, Ruoxiang; Zhu, Shisheng; Wang, Yaping"

作者地址："[Hou, Jiying; Wang, Yaping] Chongqing Med Univ, Dept Histol & Embryol, Lab Stem Cells & Tissue Engn, Chongqing 400016, Peoples R China; [Ma, Ruoxiang; Zhu, Shisheng] Chongqing Med & Pharmaceut Coll, Fac Basic Med Sci, Chongqing 401331, Peoples R China"

通信作者："Wang, YP (通讯作者)，Chongqing Med Univ, Dept Histol & Embryol, Lab Stem Cells & Tissue Engn, Chongqing 400016, Peoples R China.; Zhu, SS (通讯作者)，Chongqing Med & Pharmaceut Coll, Fac Basic Med Sci, Chongqing 401331, Peoples R China."

来源：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ESI学科分类：CHEMISTRY

WOS号：WOS:000852566300001

JCR分区：Q2

影响因子：5.6

年份：2022

卷号：23

期号：17

开始页：　

结束页：　

文献类型：Article

关键词：ginsenoside Rg1; 4D-label-free proteomic analysis; oxidative stress; hepatic metabolism; D-galactose

摘要："Ginsenoside Rg1 is an important active substance isolated from the root of ginseng. In previous studies, Rg1 has shown excellent therapeutic effects in antioxidant, anti-inflammatory, and metabolic modulation. However, the therapeutic targets of Rg1 are still unknown. In this study, we investigated the therapeutic effects of Rg1 on oxidative stress-related liver damage. The oxidative stress damage model was achieved by intraperitoneal injection of D-galactose (D-gal) for 42 consecutive days in C57BL/6J mice. Rg1 treatment started on Day 16. Body weight, liver weight, degree of hepatic oxidative stress damage, serum lipid levels, and hepatic lipid and glucose metabolism were measured. Proteomics analysis was used to measure liver protein expression. The differential expression proteins were analyzed with bioinformatics. The results showed that Rg1 treatment attenuated liver damage from oxidative stress, reduced hepatic fat accumulation, promoted hepatic glycogen synthesis, and attenuated peripheral blood low-density lipoprotein (LDL), cholesterol (CHO), and triglycerides (TG) levels. Proteomic analysis suggested that Rg1 may regulate hepatocyte metabolism through ECM-Receptor, the PI3K-AKT pathway. The epidermal growth factor receptor (EGFR) and activator of transcription 1 (STAT1) may be the key protein. In conclusion, this study provides an experimental basis for further clarifying the specific mechanism of Rg1 in the treatment of oxidative stress damage-related liver disease."

基金机构："National Natural Science Foundation of China (National Natural Science Foundation of China) [81873103, 81673748]; Basic and Frontier Technological Research Program of Chongqing (Chongqing government) [cstc2019jcyj-msxmX0455]; Scientific and Technological Research Program of Chongqing Municipal Education Commission (Chongqing government) [KJQN201902804, KJQN201902805]; Scientific and Technological Research Program of Chongqing Medical and Pharmaceutical College (Chongqing Medical and Pharmaceutical College) [ygz2018103, ygz2019308, ygz2020102, ygz2021119, ygz2021124]; Innovative Research Groups of Chongqing Medical and Pharmaceutical College (Chongqing Medical and Pharmaceutical College) [YGZ2019402]; Joint Medical Research of Chongqing Science and Technology Bureau and Chongqing Municipal Health Commission (Chongqing government) [2019MSXM033]"

基金资助正文："This study was supported by grants from the National Natural Science Foundation of China (No. 81873103, 81673748) (supported by the National Natural Science Foundation of China), the Basic and Frontier Technological Research Program of Chongqing (cstc2019jcyj-msxmX0455) (supported by Chongqing government), the Scientific and Technological Research Program of Chongqing Municipal Education Commission (KJQN201902804; KJQN201902805) (supported by Chongqing government), the Scientific and Technological Research Program of Chongqing Medical and Pharmaceutical College (ygz2018103,ygz2019308,ygz2020102,ygz2021119, ygz2021124) (supported by Chongqing Medical and Pharmaceutical College), the Innovative Research Groups of Chongqing Medical and Pharmaceutical College (YGZ2019402) (supported by Chongqing Medical and Pharmaceutical College), the Joint Medical Research of Chongqing Science and Technology Bureau and Chongqing Municipal Health Commission (2019MSXM033) (supported by Chongqing government)."