Simultaneous determination of plasma indoles by HPLC with fluorescence detection: Application in polycystic ovary syndrome patients with/without depression

作者全名："Ke Yang; Ying Chen; Zhang Xiaoqing; Zhen, Qianna; Gui, Wenwu; Ban, Yanna; He, Yifan; Pan, Shengnan; Min Ding"

作者地址："[Ke Yang; Zhang Xiaoqing; Pan, Shengnan; Min Ding] Chongqing Med Univ, Sch Lab Med, Key Lab Lab Med Diagnost, Minist Educ, Chongqing, Peoples R China; [Ying Chen; Gui, Wenwu; Ban, Yanna] Chongqing Med Univ, Reprod Med Ctr, Affiliated Hosp 1, Chongqing, Peoples R China; [Zhen, Qianna; He, Yifan] Chongqing Med Univ, Dept Endocrinol, Affiliated Hosp 1, Chongqing, Peoples R China"

通信作者："Min, D (通讯作者)，Chongqing Med Univ, Sch Lab Med, Chongqing 400016, Peoples R China."

来源：JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES

ESI学科分类：CHEMISTRY

WOS号：WOS:000856125200001

JCR分区：Q4

影响因子：1.3

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Depression; HPLC with fluorescence detection; indoles; plasma; polycystic ovary syndrome

摘要："Microbiota-derived indoles were closely associated with psychological disorders like depression. We aimed to develop an HPLC method with fluorescence detection (FLD) for simultaneous determination of plasma indoles, including indoxyl sulfate (3-INDS), indole-3-acetic acid (IAA), indole-3-propionate (IPA), indole (IND) and 3-methylindole (3-MI). Diethyl ether and ethyl acetate were selected as the mixed extractant for the sample preparation. The separation was carried out on a Shim-Pack VP-ODS column (150 x 4.6 mm, 4.6 mu m) with the mobile phase composed of 10 mmol/L sodium dihydrogen phosphate/methanol (40:60, v/v). The excitation and emission wavelengths were set at 280 and 355 nm, respectively. The linearities in plasma were obtained in the range of 1.56-400.0 mu mol/L for 3-INDS, 0.312-10.0 mu mol/L for IAA, 0.125-6.00 mu mol/L for IPA, 6.25-400.0 nmol/L for IND, and 1.56-400.0 nmol/L for 3-MI, respectively. The coefficients of variation (CVs) for each analyte of the intra-day and inter-day precisions were within 4.0 and 5.2%, respectively. The recoveries were in the range of 90.1-109.3%. This sensitive and accurate method was applied to analyze plasma indoles for the diagnostic of depression in the patients with polycystic ovary syndrome (PCOS)."

基金机构：Natural Science Foundation of Chongqing [cstc2019jcyj-msxmX0103]; Science and Technology Research Program of Chongqing Municipal Education Commission [KJQN201800437]; Chongqing Health Commission [2019MSXM060]

基金资助正文："This work was supported by the Natural Science Foundation of Chongqing (cstc2019jcyj-msxmX0103), the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No. KJQN201800437), and Chongqing Health Commission (2019MSXM060)."