Flavonoid Baicalein Suppresses Oral Biofilms and Protects Enamel Hardness to Combat Dental Caries

作者全名："Chen, Hong; Xie, Sihong; Gao, Jing; He, Liwen; Luo, Wenping; Tang, Yunhao; Weir, Michael D.; Oates, Thomas W.; Xu, Hockin H. K.; Yang, Deqin"

作者地址："[Chen, Hong; Xie, Sihong; Gao, Jing; He, Liwen; Luo, Wenping; Yang, Deqin] Chongqing Med Univ, Dept Endodont, Stomatol Hosp, Chongqing 404100, Peoples R China; [Chen, Hong; Xie, Sihong; Gao, Jing; He, Liwen; Luo, Wenping; Yang, Deqin] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing 404100, Peoples R China; [Chen, Hong; Xie, Sihong; Gao, Jing; He, Liwen; Luo, Wenping; Yang, Deqin] Chongqing Municipal Key Lab Oral Biomed Engn High, Chongqing 404100, Peoples R China; [Chen, Hong; Gao, Jing; He, Liwen; Luo, Wenping; Yang, Deqin] Chongqing Key Lab Oral Dis & Biomed Sci, 426 Songshi North Rd, Chongqing 401147, Peoples R China; [Tang, Yunhao; Weir, Michael D.; Oates, Thomas W.; Xu, Hockin H. K.] Univ Maryland, Dept Adv Oral Sci & Therapeut, Dent Sch, Baltimore, MD 21201 USA; [Xu, Hockin H. K.] Univ Maryland, Ctr Stem Cell Biol & Regenerat Med, Sch Med, Baltimore, MD 21201 USA; [Xu, Hockin H. K.] Univ Maryland, Marlene & Stewart Greenebaum Canc Ctr, Sch Med, Baltimore, MD 21201 USA"

通信作者："Yang, DQ (通讯作者)，Chongqing Med Univ, Dept Endodont, Stomatol Hosp, Chongqing 404100, Peoples R China.; Yang, DQ (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing 404100, Peoples R China.; Yang, DQ (通讯作者)，Chongqing Municipal Key Lab Oral Biomed Engn High, Chongqing 404100, Peoples R China.; Yang, DQ (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, 426 Songshi North Rd, Chongqing 401147, Peoples R China."

来源：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ESI学科分类：CHEMISTRY

WOS号：WOS:000856445200001

JCR分区：Q2

影响因子：5.6

年份：2022

卷号：23

期号：18

开始页：　

结束页：　

文献类型：Article

关键词：baicalein; Streptococcus mutans; Candida albicans; biofilm inhibition; dental caries

摘要："The objectives of this study were to investigate the effects of a novel method using flavonoids to inhibit Streptococcus mutans (S. mutans), Candida albicans (C. albicans) and dual-species biofilms and to protect enamel hardness in a biofilm-based caries model for the first time. Several flavonoids, including baicalein, naringenin and catechin, were tested. Gold-standard chlorhexidine (CHX) and untreated (UC) groups served as controls. Optimal concentrations were determined by cytotoxicity assay. Biofilm MTT, colony-forming-units (CFUs), biofilm biomass, lactic acid and polysaccharide production were evaluated. Real-time-polymerase-chain reaction (qRT-PCR) was used to determine gene expressions in biofilms. Demineralization of human enamel was induced via S. mutans-C. albicans biofilms, and enamel hardness was measured. Compared to CHX and UC groups, the baicalein group achieved the greatest reduction in S. mutans, C. albicans and S. mutans-C. albicans biofilms, yielding the least metabolic activity, polysaccharide synthesis and lactic acid production (p < 0.05). The biofilm CFU was decreased in baicalein group by 5 logs, 4 logs, 5 logs, for S. mutans, C. albicans and S. mutans-C. albicans biofilms, respectively, compared to UC group. When tested in a S. mutans-C. albicans in vitro caries model, the baicalein group substantially reduced enamel demineralization under biofilms, yielding an enamel hardness that was 2.75 times greater than that of UC group. Hence, the novel baicalein method is promising to inhibit dental caries by reducing biofilm formation and protecting enamel hardness."

基金机构："National Natural Science Foundation of China [31970783, 82100991]; Program for Top talent Distinguished Professor from Chongqing Medical University; Program for Youth Innovation in Future Medicine from Chongqing Medical University [W0060]; Science and Technology Research Program of Chongqing Municipal Education Commission [KJQN201900401]; Chongqing Special Postdoctoral Science Foundation [2010010005994583]"

基金资助正文："This study was supported by National Natural Science Foundation of China (No. 31970783 to D.Y., No. 82100991 to H.C.); Program for Top talent Distinguished Professor from Chongqing Medical University to D.Y., Program for Youth Innovation in Future Medicine from Chongqing Medical University (No. W0060 to D.Y.); the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No. KJQN201900401 to Y.T.) and Chongqing Special Postdoctoral Science Foundation (No. 2010010005994583 to H.C.)."