New Insight into Assembled Fe3O4@PEI@Ag Structure as Acceptable Agent with Enzymatic and Photothermal Properties

作者全名："Wang, Teng; Hu, Xi; Yang, Yujun; Wu, Qing; He, Chengdian; He, Xiong; Wang, Zhenyu; Mao, Xiang"

作者地址："[Wang, Teng; Hu, Xi; Wu, Qing; He, Chengdian; He, Xiong; Wang, Zhenyu; Mao, Xiang] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China; [Wang, Teng; Hu, Xi; Wu, Qing; He, Chengdian; He, Xiong; Wang, Zhenyu; Mao, Xiang] Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China; [Yang, Yujun] Chongqing Med Univ, Chongqing Med Lab Microfluid & SPRi Engn Res Ctr, Dept Lab Med, Key Lab Lab Med Diagnost,Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Wang, ZY; Mao, X (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China.; Wang, ZY; Mao, X (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China."

来源：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ESI学科分类：CHEMISTRY

WOS号：WOS:000856461700001

JCR分区：Q2

影响因子：5.6

年份：2022

卷号：23

期号：18

开始页：　

结束页：　

文献类型：Article

关键词：Fe3O4@PEI@Ag; assembling process; enzymatic; catalytic performance; photothermal property

摘要："Metal-based enzyme mimics are considered to be acceptable agents in terms of their biomedical and biological properties; among them, iron oxides (Fe3O4) are treated as basement in fabricating heterogeneous composites through variable valency integrations. In this work, we have established a facile approach for constructing Fe3O4@Ag composite through assembling Fe3O4 and Ag together via polyethyleneimine ethylenediamine (PEI) linkages. The obtained Fe3O4@PEI@Ag structure conveys several hundred nanometers (similar to 150 nm). The absorption peak at 652 nm is utilized for confirming the peroxidase-like activity of Fe3O4@PEI@Ag structure by catalyzing 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2. The Michaelis-Menten parameters (K-m) of 1.192 mM and 0.302 mM show the higher catalytic activity and strong affinity toward H2O2 and TMB, respectively. The maximum velocity (V-max) value of 1.299 x 10(-7) M.s(-1) and 1.163 x 10(-7) M.s(-1) confirm the efficiency of Fe3O4@PEI@Ag structure. The biocompatibility illustrates almost 100% cell viability. Being treated as one simple colorimetric sensor, it shows relative selectivity and sensitivity toward the detection of glucose based on glucose oxidase. By using indocyanine green (ICG) molecule as an additional factor, a remarkable temperature elevation is observed in Fe3O4@PEI@Ag@ICG with increments of 21.6 degrees C, and the absorption peak is nearby 870 nm. This implies that the multifunctional Fe3O4@PEI@Ag structure could be an alternative substrate for formatting acceptable agents in biomedicine and biotechnology with enzymatic and photothermal properties."

基金机构："Science and Technology Research program of Chongqing Municipal Education Commission [KJQN201900436, KJQN202000420]; Science and Technology Planning Project of Yuzhong District, Chongqing, China [20200118]; National Natural Science Foundation of China [81501617]"

基金资助正文："This work was supported by the Science and Technology Research program of Chongqing Municipal Education Commission (Grants no. KJQN201900436, KJQN202000420), the Science and Technology Planning Project of Yuzhong District, Chongqing, China (Grants no.: 20200118), the National Natural Science Foundation of China (Grants no.: 81501617)."