Repair of Large-Scale Rib Defects Based on Steel-Reinforced Concrete-Designed Biomimetic 3D-Printed Scaffolds with Bone-Mineralized Microenvironments

作者全名："Bai, Baoshuai; Hao, Junxiang; Hou, Mengjie; Wang, Tao; Wu, Xiaodi; Liu, Yanhan; Wang, Yiyang; Dai, Chengxiang; Hua, Yujie; Ji, Guangyu; Zhou, Guangdong"

作者地址："[Bai, Baoshuai; Hou, Mengjie; Hua, Yujie; Zhou, Guangdong] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Shanghai Key Lab Tissue Engn, Dept Plast & Reconstruct Surg,Sch Med, Shanghai 200001, Peoples R China; [Bai, Baoshuai; Hao, Junxiang; Wang, Tao; Wu, Xiaodi] Weifang Med Univ, Res Inst Plast Surg, Weifang 261000, Shandong, Peoples R China; [Hao, Junxiang; Hou, Mengjie; Wang, Tao; Wu, Xiaodi; Wang, Yiyang; Hua, Yujie; Ji, Guangyu; Zhou, Guangdong] Natl Tissue Engn Ctr China, Shanghai 200001, Peoples R China; [Liu, Yanhan; Hua, Yujie; Ji, Guangyu; Zhou, Guangdong] Shanghai Jiao Tong Univ, Sch Med, Shanghai 200240, Peoples R China; [Dai, Chengxiang] Cellular Biomed Grp Inc, Shanghai 201210, Peoples R China; [Ji, Guangyu] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Dept Thorac Surg, Shanghai 200001, Peoples R China; [Wang, Yiyang] Chongqing Med Univ, Affiliated Hosp 3, Dept Orthoped, Chongqing 401120, Peoples R China"

通信作者："Hua, YJ; Zhou, GD (通讯作者)，Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Shanghai Key Lab Tissue Engn, Dept Plast & Reconstruct Surg,Sch Med, Shanghai 200001, Peoples R China.; Hua, YJ; Ji, GY; Zhou, GD (通讯作者)，Natl Tissue Engn Ctr China, Shanghai 200001, Peoples R China.; Hua, YJ; Ji, GY; Zhou, GD (通讯作者)，Shanghai Jiao Tong Univ, Sch Med, Shanghai 200240, Peoples R China.; Dai, CX (通讯作者)，Cellular Biomed Grp Inc, Shanghai 201210, Peoples R China.; Ji, GY (通讯作者)，Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Dept Thorac Surg, Shanghai 200001, Peoples R China."

来源：ACS APPLIED MATERIALS & INTERFACES

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000857293200001

JCR分区：Q1

影响因子：9.5

年份：2022

卷号：14

期号：37

开始页：42388

结束页：42401

文献类型：Article

关键词：tissue engineering; 3D-printed scaffold; rib regeneration; osteogenic inorganic salts; mechanical analysis

摘要："Tissue engineering technology provides a promising approach for large-scale bone reconstruction in cases of extensive chest wall defects. However, previous studies did not consider meticulous scaffold design specific to large-scale rib regeneration in terms of three-dimensional (3D) shape, proper porous structures, enough mechanical strength, and osteogenic microenvironments. Thus, there is an urgent need to develop an appropriate bone biomimetic scaffold (BBS) to address this problem. In this study, a BBS with controllable 3D morphology, appropriate mechanical properties, good biocompatibility and biodegradability, porous structure suitable for cell loading, and a biomimetic osteogenic inorganic salt (OIS) microenvironment was successfully prepared by integrating computer -aided design, 3D-printing, cast-molding, and freeze-drying technologies. The addition of the OIS in the scaffold substantially promoted ectopic bone regeneration in vivo, which might be attributed to the activation of osteogenic and angiogenic signaling pathways as well as upregulated expression of osteogenic genes. More importantly, dual long rib defects could be successfully repaired and medullary cavity recanalized by the rib-shaped mature cortical bone, which might be mediated by the activation of osteoclast signaling pathways. Thus, this paper presents a reliable BBS and proposes a new strategy for the repair of large-scale bone defects."

基金机构："National Key Research and Development Program of China [2018YFC1105803, 2017YFC1103900]; National Natural Science Foundation of China [81871502, 81701843, 81671837]; Program of Shanghai Academic/Technology Research Leader [19XD1431100]; Shanghai Collaborative Innovation Program on Regenerative Medicine and Stem Cell Research [2019CXJQ01]; Clinical Research Plan of SHDC [SHDC2020CR2045B]"

基金资助正文："This research was financially supported by the National Key Research and Development Program of China (2018YFC1105803, 2017YFC1103900) , the National Natural Science Foundation of China (81871502, 81701843, and 81671837) , the Program of Shanghai Academic/Technology Research Leader (19XD1431100) , the Shanghai Collaborative Innovation Program on Regenerative Medicine and Stem Cell Research (2019CXJQ01) , and the Clinical Research Plan of SHDC (No. SHDC2020CR2045B) ."