Antitumor effect of a polysaccharide from Pseudostellaria heterophylla through reversing tumor-associated macrophages phenotype

作者全名："Pu, Youwei; Zhu, Junmo; Xu, Jie; Zhang, Sitong; Bao, Yixi"

作者地址："[Pu, Youwei; Zhu, Junmo; Xu, Jie; Zhang, Sitong; Bao, Yixi] Chongqing Med Univ, Dept Clin Lab, Affiliated Hosp 2, Chongqing 400010, Peoples R China; [Bao, Yixi] Chongqing Med Univ, Dept Clin Lab, Affiliated Hosp 2, 76 Linjiang Rd, Chongqing 400010, Peoples R China"

通信作者："Bao, YX (通讯作者)，Chongqing Med Univ, Dept Clin Lab, Affiliated Hosp 2, 76 Linjiang Rd, Chongqing 400010, Peoples R China."

来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000858845200004

JCR分区：Q1

影响因子：8.2

年份：2022

卷号：220

期号：　

开始页：816

结束页：826

文献类型：Article

关键词：Pseudostellaria heterophylla; Macrophage polarization; Immunomodulatory effect

摘要："Tumor-associated macrophages (TAMs), which are predominant tumor-infiltrating immune cells in the tumor microenvironment, participate in promoting the occurrence and metastasis of tumor cells. Reprogramming TAMs has become a promising immunotherapeutic approach for novel cancer treatments. In this study, a homogeneous polysaccharide (PHP-1) was obtained from Pseudostellaria heterophylla, and its antitumor and immunological activities, as well as the underlying molecular mechanisms were explored. These findings suggested that PHP-1 can switch M2 macrophages to the M1 type, thereby promoting tumor cell apoptosis in vitro. In addition, PHP-1 can modulate the TAMs phenotype, maintain the CD4+/CD8+ lymphocyte balance, and exert antitumor effects in H22 tumor-bearing mice. Mechanistically, PHP-1 is recognized by the TLR4 receptor, promotes Ca2+ release, and activates the NF-kappa B and MAPK signaling pathways to reset the M2-type macrophages. These findings indicate that PHP-1 from P. heterophylla can function as a tumor immunotherapeutic modulator."

基金机构：Natural Science Foundation of Chongqing of China [cstc2021jcyj-msxmX0094]; National Natural Science Foundation of China [81773940]

基金资助正文：Acknowledgements This work was supported by the Natural Science Foundation of Chongqing of China (No. cstc2021jcyj-msxmX0094) and the National Natural Science Foundation of China (No. 81773940) .