Salivary orosomucoid 1 as a biomarker of hepatitis B associated hepatocellular carcinoma

作者全名："He, Jiaoxia; Zheng, Zhongling; Liu, Tingting; Ao, Yupei; Yang, Yixuan; Hu, Huaidong"

作者地址："[He, Jiaoxia; Zheng, Zhongling; Liu, Tingting; Ao, Yupei; Yang, Yixuan; Hu, Huaidong] Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Dept Infect Dis,Key Lab Mol Biol Infect Dis,Minis, Chongqing 400000, Peoples R China"

通信作者："Hu, HD (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Dept Infect Dis,Key Lab Mol Biol Infect Dis,Minis, Chongqing 400000, Peoples R China."

来源：SCIENTIFIC REPORTS

ESI学科分类：Multidisciplinary

WOS号：WOS:000859184900054

JCR分区：Q1

影响因子：4.6

年份：2022

卷号：12

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Saliva is rich in proteins, DNA, RNA and microorganisms, and can be regarded as a biomarker library. In order to explore a noninvasive and simple means of early screening for liver cancer, proteomics was used to screen salivary markers of hepatitis B associated liver cancer. We used mass spectrometry coupled isobaric tags for relative and absolute quantitation (iTRAQ)-technology to identify differentially expressed proteins (DEPs). Western blot, immunohistochemistry and enzyme linked immunosorbent assay were used to detect marker expression of in tissues and saliva. Statistical analysis was used to analyze the diagnostic efficacy of the markers was analyzed through statistical analyses. By comparing the hepatocellular carcinoma (HCC) group with non-HCC groups, we screened out 152 salivary DEPs. We found orosomucoid 1(ORM1) had significantly higher expression in saliva of HCC patients compared with non-HCC groups (p < 0.001) and the expression of ORM1 in liver cancer tissues was significantly higher than that in adjacent normal tissues (p < 0.001). The combination of salivary ORM1 and alpha-fetoprotein (AFP) showed reasonable specificities and sensitivities for detecting HCC. In a word, salivary ORM1 as a new biomarker of hepatitis B associated hepatocellular carcinoma, combination of salivary ORM1 and AFP as an improved diagnostic tool for hepatocellular carcinoma."

基金机构：Natural Science Foundation of China [81171560]; "Par-Eu Scholars Program" of Chongqing City; National Science and Technology Major Project of China [2012ZX10002007001]

基金资助正文："We thank the individuals that consented to be included in the cohorts of this project. The study was supported by the Natural Science Foundation of China (grant no. 81171560) and the ""Par-Eu Scholars Program"" of Chongqing City, the National Science and Technology Major Project of China (Grant No. 2012ZX10002007001)."