Periodontitis induced by orthodontic wire ligature drives oral microflora dysbiosis and aggravates alveolar bone loss in an improved murine model

作者全名："Ai, Rongshuang; Li, Dingyi; Shi, Luyao; Zhang, Xiaonan; Ding, Zhiqiang; Zhu, Yiting; He, Yujuan"

作者地址："[Ai, Rongshuang; Li, Dingyi; Shi, Luyao; Zhu, Yiting; He, Yujuan] Chongqing Med Univ, Dept Lab Med, Key Lab Diagnost Med, Minist Educ, Chongqing, Peoples R China; [Zhang, Xiaonan] Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China; [Ding, Zhiqiang] Chongqing Inst Engn, Sch Comp Sci, Chongqing, Peoples R China; [Zhu, Yiting] Chongqing Univ, Dept Lab Med, Gorges Hosp 3, Chongqing, Peoples R China"

通信作者："He, YJ (通讯作者)，Chongqing Med Univ, Dept Lab Med, Key Lab Diagnost Med, Minist Educ, Chongqing, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000859673600001

JCR分区：Q2

影响因子：5.2

年份：2022

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：orthodontic wire; ligation; inflammation; alveolar bone loss; 16S rRNA; microbiota dysbiosis

摘要："Aim To assess the contribution of polymicrobial disruption of host homeostasis to periodontitis progression in orthodontic wire ligation murine model. Methods Orthodontic wire rings were inserted between the first and second molars of mice for 18 days for the orthodontic wire ligation mouse model, and Pg injection model and Pg-LPS injection model were used as controls. Alveolar bone loss and periodontal inflammation were analyzed by micro-CT, histological staining and qRT-PCR. Further, pyrosequencing of 16S rRNA gene amplicon was used to analyze the development of oral microorganism dysbiosis in the mice. Results Micro-CT, TRAP staining and qRT-PCR showed that orthodontic wire ligation model led to more severe alveolar bone loss than Pg and Pg-LPS models. H&E staining and qRT-PCR demonstrated that stronger inflammatory response was induced by the orthodontic wire treatment compared to the other models. In addition, pyrosequencing of 16S rRNA gene amplicons revealed that the composition of oral microbiota presented a transition as the disease progressed and significant differences emerged in oral microbiota communities between orthodontic ligature mice and healthy controls. Furthermore, antibiotic treatment decreased both inflammation and alveolar bone loss in response to microbial community dysbiosis. However, no significant difference in bacterial community composition was observed in Pg and Pg-LPS models. Conclusions Orthodontic wire ligation drove oral microbial community transitions that mimicked polymicrobial communities characterized by polymicrobial synergy and dysbiosis. Our improved model is suitable for further study of pathogenesis of periodontitis and exploration of corresponding treatment strategies."

基金机构：Chongqing Research Program of Basic Research and Frontier Technology [cstc2018jcyjAX0257]

基金资助正文：This work was supported by Chongqing Research Program of Basic Research and Frontier Technology (No. cstc2018jcyjAX0257).