Sensitive detection of MiRNA and CircRNA through DSN enzyme cooperating NEase assisted dual signal amplification

作者全名："Zhao, Gang; Yan, Xiaohang; Zhang, Yi; Deng, Jing; Liang, Xiaojun"

作者地址："[Zhao, Gang; Zhang, Yi] Army Med Univ, Xinqiao Hosp, Dept Radiol, Chongqing 400038, Peoples R China; [Yan, Xiaohang] Chongqing Kanghua Zhonglian Cardiovasc Hosp, Dept Radiol, Chongqing 400025, Peoples R China; [Deng, Jing] Chongqing Med Univ, Dept Radiol, Affiliated Hosp 3, Chongqing 400016, Peoples R China; [Liang, Xiaojun] Army Med Univ, Xinqiao Hosp, Dept Otorhinolaryngol, Chongqing 400038, Peoples R China; [Liang, Xiaojun] Army Med Univ, Xinqiao Hosp, Dept Otorhinolaryngol, Chongqing 400038, Peoples R China"

通信作者："Liang, XJ (通讯作者)，Army Med Univ, Xinqiao Hosp, Dept Otorhinolaryngol, Chongqing 400038, Peoples R China."

来源：ANALYTICAL BIOCHEMISTRY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000860475500008

JCR分区：Q2

影响因子：2.9

年份：2022

卷号：654

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：DSN enzyme; miRNA; NEase; circRNA

摘要："MicroRNA (miRNA) and circular RNA (circRNA) are promising biomarkers for early-diagnosis of a variety of diseases, such as myocardial infarction and cancers. However, few methods reported simultaneous and sensitive detection of multiple miRNAs. Herein, we design a novel approach with an improved miRNA and circRNA detection sensitivity. There are only two probes designed with hairpin probe, namely catch probe and report probe, in the system. When target miRNA or circRNA existed, it can unfold catch probe through hybridizing with toehold section and form a rigid DNA-RNA duplex. The DNA sequence in the formed duplex is identified and digested by duplex-specific nuclease (DSN enzyme), and consequently, target miRNA is released to attend a next signal cycle. The rested DNA sequence in catch probe (initiator probe) recognizes report probe and separates its hairpin structure to generate fluorescence signals, forming a double-strand DNA (dsDNA) product. Unfold report probe sequence in the dsDNA product is then digested by DNA nicking endonuclease (NEase) and initiator is released to trigger amplified signals. Based on the DSN enzyme cooperating NEase assisted dual signal amplification, the method exhibits a greatly improved detection sensitivity."

基金机构：Natural Science Foundation of Army Medical University [2018JSLC0024]

基金资助正文：Acknowledgements The research is funded by Natural Science Foundation of Army Medical University (No. 2018JSLC0024) .