Genetically engineered bacteria-mediated multi-functional nanoparticles for synergistic tumor-targeting therapy

作者全名："Wang, Yaotai; Tang, Yu; Du, Yan; Lin, Li; Zhang, Zhong; Ou, Xia; Chen, Sheng; Wang, Qi; Zou, Jianzhong"

作者地址："[Wang, Yaotai; Tang, Yu; Du, Yan; Lin, Li; Zhang, Zhong; Ou, Xia; Chen, Sheng; Wang, Qi; Zou, Jianzhong] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, 40 0 016, Chongqing, Peoples R China; [Wang, Yaotai; Tang, Yu; Wang, Qi; Zou, Jianzhong] Chongqing Med Univ, Chongqing Key Lab Biomed Engn, 40 0 016, Chongqing, Peoples R China; [Zou, Jianzhong] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China"

通信作者："Zou, JZ (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China."

来源：ACTA BIOMATERIALIA

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000860679800008

JCR分区：Q1

影响因子：9.7

年份：2022

卷号：150

期号：　

开始页：337

结束页：352

文献类型：Article

关键词：Tumor therapy; Focused ultrasound ablation surgery; Bacteriotherapy; Hypoxic activation; Multimodal imaging

摘要："Focused ultrasonic ablation surgery (FUAS) for tumor treatment has emerged as an effective non-invasive therapeutic approach, but its widespread clinical utilization is limited by its low therapeutic efficiency caused by inadequate tumor targeting, single imaging modality, and possible tumor recurrence following surgery. Therefore, this study aimed to develop a biological targeting synergistic system consisting of genetically engineered bacteria and multi-functional nanoparticles to overcome these limitations. Escherichia coli was genetically modified to carry an acoustic reporter gene encoding the formation of gas vesicles (GVs) and then target the tumor hypoxic environment in mice. After E. coli producing GVs (GVs- E. coli ) colonized the tumor target area, ultrasound imaging and collaborative FUAS were performed; multifunctional nanoparticles were then enriched in the tumor target area through electrostatic adsorption. Multi-functional cationic lipid nanoparticles containing IR780, perfluorohexane, and banoxantrone dihydrochloride (AQ4N) were coloaded in the tumor to realize targeted multimodal imaging and enhance the curative effect of FUAS. AQ4N was stimulated by the tumor hypoxic environment and synergistically cooperated with FUAS to kill tumor cells. In sum, synergistic tumor therapy involving multi-functional nanoparticles mediated by genetically engineered bacteria overcomes the limitations and improves the curative effect of existing FUAS."

基金机构：Chongqing Graduate Scientific Research Innovation Project [CYB21194]

基金资助正文：This work was supported by the Chongqing Graduate Scientific Research Innovation Project (CYB21194) .