Eupalinolide A induces autophagy via the ROS/ERK signaling pathway in hepatocellular carcinoma cells in vitro and in vivo

作者全名："Zhang, Yonghui; Dong, Feng; Cao, Zhihao; Wang, Tingting; Pan, Lian; Luo, Wujing; Ding, Wenxuan; Li, Jiaxin; Jin, Lishan; Liu, Huan; Zhang, Haoyang; Mu, Jinage; Han, Meiyue; Wei, Yong; Deng, Xuesong; Liu, Dan; Hao, Po; Zeng, Gang; Pang, Yi; Liu, Guiyuan; Zhen, Changlin"

作者地址："[Zhang, Yonghui; Dong, Feng; Cao, Zhihao; Wang, Tingting; Pan, Lian; Luo, Wujing; Ding, Wenxuan; Li, Jiaxin; Jin, Lishan; Liu, Huan; Zhang, Haoyang; Mu, Jinage; Han, Meiyue; Deng, Xuesong; Liu, Dan; Hao, Po; Zeng, Gang; Pang, Yi; Liu, Guiyuan; Zhen, Changlin] Chongqing Key Lab Dev & Utilizat Genuine Med Mat T, Chongqing 404120, Peoples R China; [Zhang, Yonghui; Dong, Feng; Cao, Zhihao; Wang, Tingting; Pan, Lian; Luo, Wujing; Ding, Wenxuan; Li, Jiaxin; Jin, Lishan; Liu, Huan; Zhang, Haoyang; Mu, Jinage; Han, Meiyue; Deng, Xuesong; Liu, Dan; Hao, Po; Zeng, Gang; Pang, Yi; Liu, Guiyuan; Zhen, Changlin] Chongqing Three Gorges Med Coll, Chongqing Engn Res Ctr Antitumor Nat Drugs, Chongqing 404120, Peoples R China; [Zhang, Yonghui] Chongqing Med Univ, Hepatol Surg Dept, Affiliated Hosp 1, Chongqing 400042, Peoples R China; [Wei, Yong] Chongqing Three Gorges Univ, Coll Elect & Informat Engn, Key Lab Intelligent Informat Proc & Control, Chongqing 404110, Peoples R China; [Liu, Guiyuan] Chongqing Three Gorges Med Coll, Affiliated Hosp, Gen Surg Dept, Chongqing 404000, Peoples R China; [Liu, Guiyuan] Chongqing Three Gorges Med Coll, Affiliated Hosp, Gen Surg Dept, 38 Guanmenshi Rd, Chongqing 404000, Peoples R China; [Zhen, Changlin] Chongqing Three Gorges Med Coll, Chongqing Key Lab Dev & Utilizat Genuine Med Mat T, 366 Tianxing Rd, Chongqing 404120, Peoples R China"

通信作者："Liu, GY (通讯作者)，Chongqing Three Gorges Med Coll, Affiliated Hosp, Gen Surg Dept, 38 Guanmenshi Rd, Chongqing 404000, Peoples R China.; Zhen, CL (通讯作者)，Chongqing Three Gorges Med Coll, Chongqing Key Lab Dev & Utilizat Genuine Med Mat T, 366 Tianxing Rd, Chongqing 404120, Peoples R China."

来源：INTERNATIONAL JOURNAL OF ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000862430900001

JCR分区：Q1

影响因子：5.2

年份：2022

卷号：61

期号：5

开始页：　

结束页：　

文献类型：Article

关键词：eupalinolide A; hepatocellular carcinoma; reactive oxygen species; autophagy; ERK

摘要："Hepatocellular carcinoma is the most common primary malignancy of the liver. The current systemic drugs used to treat hepatocellular carcinoma result in low overall survival time. It has therefore been suggested that new small-molecule drugs should be developed for treating hepatocellular carcinoma. Eupatorium lindleyanum DC. (EL) has been used to treat numerous diseases, particularly respiratory diseases; however, to the best of our knowledge, studies have not yet fully elucidated the effect of EL on hepatocellular carcinoma. In the present study, the effect of eupalinolide A (EA), one of the extracts of EL, was evaluated on tumor growth in a xenograft model of human hepatocellular carcinoma cells, and on the proliferation and migration of hepatocellular carcinoma cell lines. Cell cycle progression and the type of cell death were then evaluated using the Cell Counting Kit 8 assay, flow cytometry, electron microscopy and western blotting. EA significantly inhibited cell proliferation and migration by arresting the cell cycle at the G(1) phase and inducing autophagy in hepatocellular carcinoma cells. EA-induced autophagy was mediated by reactive oxygen species (ROS) and ERK signaling activation. Specific inhibitors of ROS, autophagy and ERK inhibited EA-induced cell death and migration. In conclusion, the present study revealed that EA may inhibit the proliferation and migration of hepatocellular carcinoma cells, highlighting its potential as a promising antitumor compound for treating hepatocellular carcinoma."

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