Melanoma stem cells promote metastasis via exosomal miR-1268a inactivation of autophagy

作者全名："Li, Xiaoshuang; Liu, Doudou; Chen, Hao; Zeng, Bin; Zhao, Qiting; Zhang, Yuhan; Chen, Yuting; Wang, Jianyu; Xing, H. Rosie"

作者地址："[Zeng, Bin; Zhao, Qiting; Wang, Jianyu] Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China; [Li, Xiaoshuang; Liu, Doudou; Chen, Hao; Zhang, Yuhan; Chen, Yuting; Xing, H. Rosie] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China; [Li, Xiaoshuang; Liu, Doudou; Chen, Hao; Zhang, Yuhan; Chen, Yuting; Xing, H. Rosie] Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China"

通信作者："Wang, JY (通讯作者)，Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China.; Xing, HR (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China."

来源：BIOLOGICAL RESEARCH

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000862562500001

JCR分区：Q1

影响因子：6.7

年份：2022

卷号：55

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Melanoma stem cells; Exosomes; miR-1268a; Metastasis; Autophagy

摘要："Background Metastatic melanoma has a high mortality rate and poor survival. This is associated with efficient metastatic colonization, but the underlying mechanisms remain elusive. Communication between cancer stem cells (CSCs) and cancer cells plays an important role in metastatic dissemination. Whether cancer stem cells can alter the metastatic properties of non-CSC cells; and whether exosomal crosstalk can mediate such interaction, have not been demonstrated in melanoma prior to this report. Results The results revealed that exosomes secreted by highly metastatic melanoma CSCs (OL-SCs) promoted the invasiveness of the low metastatic melanoma cells (OL) and accelerated metastatic progression. miR-1268a was up-regulated in cells and exosomes of OL-SCs. Moreover, OL-SCs-derived exosomal miR-1268a, upon taking up by OL cells, promoted the metastatic colonization ability of OL cells in vitro and in vivo. In addition, the pro-metastatic activity of exosomal miR-1268a is achieved through inhibition of autophagy. Conclusion Our study demonstrates that OL cells can acquire the ""metastatic ability"" from OL-SCs cells. OL-SCs cells achieves this goal by utilizing its exosomes to deliver functional miRNAs, such as miR-1268a, to the targeted OL cells which in turn augments metastatic colonization by inactivating the autophagy pathway in OL cells."

基金机构："National Natural Science Fund [82073277, 82173247]; Science and Technology Project Affiliated to the Education Department of Chongqing [KJQN202100404]; Natural Science Fund of Chongqing [cstc2019jcyj-msxmX0868]; Science and Technology Project of Chongqing Yuzhong District [20200110]"

基金资助正文："This work was supported by the National Natural Science Fund (Grant No. 82073277 and 82173247), the Science and Technology Project Affiliated to the Education Department of Chongqing (Grant No. KJQN202100404), Natural Science Fund of Chongqing (Grant No. cstc2019jcyj-msxmX0868) and Science and Technology Project of Chongqing Yuzhong District (Grant No. 20200110)."