Esomeprazole inhibits endoplasmic reticulum stress and ameliorates myocardial ischemia-reperfusion injury

作者全名："Zhou, Guoxiang; Peng, Yuce; Guo, Mingyu; Qu, Can; Luo, Suxin; Jiang, Yingjiu; Chen, Dan; Wang, Xiaowen; Guo, Yongzheng"

作者地址："[Zhou, Guoxiang; Jiang, Yingjiu; Chen, Dan; Wang, Xiaowen] Chongqing Med Univ, Affiliated Hosp 1, Dept Cardiothorac Surg, Chongqing 400016, Peoples R China; [Peng, Yuce; Guo, Mingyu; Luo, Suxin; Guo, Yongzheng] Chongqing Med Univ, Affiliated Hosp 1, Div Cardiol, Chongqing 400016, Peoples R China; [Qu, Can] Chongqing Med Univ, Affiliated Hosp 1, Dept Pharm, Chongqing 400016, Peoples R China"

通信作者："Wang, XW (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Cardiothorac Surg, Chongqing 400016, Peoples R China.; Guo, YZ (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Div Cardiol, Chongqing 400016, Peoples R China."

来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000862974600001

JCR分区：Q2

影响因子：3.1

年份：2022

卷号：627

期号：　

开始页：84

结束页：90

文献类型：Article

关键词：Proton pump inhibitors; Ischemia reperfusion (I/R) injury; Inflammation; Endoplasmic reticulum stress

摘要："Proton pump inhibitors (PPIs) are often prescribed in association with clopidogrel and aspirin to patients with myocardial infraction (MI), but their effects on heart is controversial. The purpose of this study was to investigate the effects and potential mechanism of omeprazole (OME) and esomeprazole (ESO) in myocardial ischemia reperfusion (I/R) injury. In the present study, mice were treated with OME, ESO or vehicle for 3 weeks and then subjected to myocardial I/R or sham surgery. At 1 day after surgery, echocardiography was performed to access cardiac injury. Hematoxylin and eosin (H & E) staining was performed to evaluate cardiomyocyte morphology. The IL1b was evaluated by Immunohistochemistry (IHC). Elisa was used to detect cTnt content in serum. The expression of CD86, CD206, CHOP, ATF6, eIF2a and p eIF2a were determined by Western blot (WB). The result showed that ESO markedly improved the left ventricular ejection fraction (LVEF), shortening fraction (FS), suppressed inflammatory infiltration, endoplasmic reticulum stress (ERS) and decreased proinflammatory macrophages in I/R hearts, while OME had no significant effects on cardiac function, inflammation and ERS in the I/R heart. In conclusion, ESO but not OME pretreatment reduces the proportion of proinflammatory macrophages, inhibits endoplasmic reticulum stress, and alleviates I/R injury in mice, indicating that ESO maybe a more proper PPI than OME for application in I/R injury.(c) 2022 Elsevier Inc. All rights reserved."

基金机构："High-Level Medical Training Project of the First Affiliated Hospital of Chongqing Medical University [XKTS176]; Cultivating fund of The First Affiliated Hospital of Chongqing Medical university [PYJJ2021-05, CYYY-BSHPYXM-202204]"

基金资助正文："This work was supported by the High-Level Medical Training Project of the First Affiliated Hospital of Chongqing Medical University (XKTS176) and Cultivating fund of The First Affiliated Hospital of Chongqing Medical university (PYJJ2021-05, CYYY-BSHPYXM-202204) ."