Baicalin Alleviates Contrast-Induced Acute Kidney Injury Through ROS/NLRP3/Caspase-1/GSDMD Pathway-Mediated Proptosis in vitro
作者全名:"Li, Yanyan; Wang, Junda; Huang, Dan; Yu, Chao"
作者地址:"[Li, Yanyan; Yu, Chao] Chongqing Med Univ, Coll Pharm, Chongqing, Peoples R China; [Li, Yanyan; Wang, Junda; Huang, Dan] Chongqing Tradit Chinese Med Hosp, Chongqing, Peoples R China; [Yu, Chao] Chongqing Med Univ, Coll Pharm, Yixueyuan Rd, Chongqing 400016, Peoples R China"
通信作者:"Yu, C (通讯作者),Chongqing Med Univ, Coll Pharm, Yixueyuan Rd, Chongqing 400016, Peoples R China."
来源:DRUG DESIGN DEVELOPMENT AND THERAPY
ESI学科分类:PHARMACOLOGY & TOXICOLOGY
WOS号:WOS:000863440700001
JCR分区:Q1
影响因子:4.8
年份:2022
卷号:16
期号:
开始页:3353
结束页:3364
文献类型:Article
关键词:pyroptosis; baicalin; iohexol; ROS; NLRP3; Caspase-1; GSDMD pathway; kidney injury
摘要:"Purpose: To investigate the effect of baicalin on the reactive oxygen species (ROS)/ NOD-like receptor protein 3 (NLRP3)/Caspase-1/gasdermin-D (GSDMD) inflammasome pathway and its related mechanism in regulating pyroptosis of human renal tubular epithelial cells (HK-2) induced by contrast media.Methods: Iohexol was used to act on HK-2 cells to establish a renal tubular cell pyroptosis model; and the signal pathway genes were silenced, cytokines were detected by enzyme-linked immunosorbent assay (ELISA), and cell viability, gene expression, and protein expression were evaluated by double fluorescence staining and flow cytometry. To assess the cytotoxicity caused by the contrast agent; cells were pretreated with different concentrations of baicalin; and then the cells were exposed to iohexol again, and the relevant indicators were tested again.Results: After HK-2 cells were exposed to iohexol, the NLRP3 inflammasome pathway markers NLRP3, interleukin (IL)-113, and IL-18 mRNA levels as well as the protein expression levels of NLRP3, ASC, Caspase-1, and GSDMD were up-regulated. In addition, the effect also significantly increased the IL-18, IL-113, lactate dehydrogenase (LDH), superoxide dismutase (SOD), malondialdehyde (MDA) release, and cellular ROS levels. The results of Annexin V-FITC/PI flow cytometry showed that the level of apoptosis was increased. However, after the intervention of baicalin, the changes in the above indexes caused by iohexol stimulation of HK-2 cells were inhibited.Conclusion: Exposure to iohexol can induce pyroptosis of HK-2 cells through the ROS/NLRP3/Caspase-1/GSDMD signaling pathway. Baicalin ameliorated iohexol-induced pyroptosis in HK-2 cells by regulating the NLRP3 inflammasome pathway."
基金机构:"Natural Science Foundation of Chongqing, China [cstc2020jcyj-msxmX0187]; Chongqing Science and health joint traditional Chinese medicine science and technology project, China [2020ZY3539]"
基金资助正文:"Natural Science Foundation of Chongqing in 2020 (cstc2020jcyj-msxmX0187), China; Chongqing Science and health joint traditional Chinese medicine science and technology project in 2020 (2020ZY3539), China."