Detection of rare CTCs by electrochemical biosensor built on quaternary PdPtCuRu nanospheres with mesoporous architectures

作者全名："Zhou, Xi; Bai, Dan; Fu, Yixin; Song, Lin; Wu, You; Chen, Kena; Li, Junjie; Yang, Yujun; Chen, Huajian; Wang, Zhongzhong; Xie, Guoming"

作者地址："[Zhou, Xi; Bai, Dan; Fu, Yixin; Song, Lin; Wu, You; Chen, Kena; Li, Junjie; Yang, Yujun; Chen, Huajian; Wang, Zhongzhong; Xie, Guoming] Chongqing Med Univ, Dept Lab Med, Key Lab Lab Med Diagnost, Chongqing Med Lab Microfluid,Minist Educ, Chongqing, Peoples R China; [Zhou, Xi; Bai, Dan; Fu, Yixin; Song, Lin; Wu, You; Chen, Kena; Li, Junjie; Yang, Yujun; Chen, Huajian; Wang, Zhongzhong; Xie, Guoming] Chongqing Med Univ, SPRi Engn Res Ctr, Chongqing, Peoples R China; [Chen, Huajian] Chongqing Univ, Chongqing Univ Cent Hosp, Chongqing Emergency Med Ctr, Sch Med, Chongqing, Peoples R China"

通信作者："Xie, GM (通讯作者)，Chongqing Med Univ, Dept Lab Med, Key Lab Lab Med Diagnost, Chongqing Med Lab Microfluid,Minist Educ, Chongqing, Peoples R China.; Xie, GM (通讯作者)，Chongqing Med Univ, SPRi Engn Res Ctr, Chongqing, Peoples R China."

来源：TALANTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000863525100005

JCR分区：Q1

影响因子：6.1

年份：2023

卷号：253

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：PdPtCuRu mesoporous nanospheres; CeMOF-Au; Ketjen Black; Dual -recognition; Electrochemical biosensor; Circulating tumor cells

摘要："Circulating tumor cells (CTCs) are promising liquid biopsy biomarkers for early cancer detection and anti-cancer therapy evaluation. The ultra-low abundance of CTCs in blood samples requires highly sensitive and accurate detection ways. In this study, we propose the design of a dual-recognition electrochemical biosensor to improve both the specificity and signal response. PdPtCuRu mesoporous nanospheres (PdPtCuRu MNSs) with excellent three dimensions (3D) nanopore structures were synthesized by one-pot method and connected to mucin 1 (MUC1) aptamer to serve as signal amplification probe. Besides, superconductive carbon black, Ketjen Black (KB), and gold nanoparticles (AuNPs) modified organometallic frame (CeMOF-Au) were combined to work as signal transducer. The characteristic branching structure of KB provides abundant contact points to load CeMOF-Au to heighten the interface electron transfer rate. In addition, AuNPs were reduced on the surface of CeMOF, which could effectively bind the capture antibody and further enhance the conductivity. Under the optimized condition, the limit of detection (LOD) of the as-constructed biosensor was less than 10 cells mL-1 for model A549 cells, and showed good specificity and accuracy in spiked serum samples. We envision the as-proposed electrochemical biosensor would alternate as a useful tool for the clinical detection of CTCs for cancer diagnosis."

基金机构：Graduate Scientific Research and Innovation Project of Chongqing; Basic Science and Cutting -edge Technology Research Projects of Chongqing Science and Technology Commission; [CYB21191]; [cstc2019jcyj- msxmX0203]

基金资助正文：This work was financially supported by the Graduate Scientific Research and Innovation Project of Chongqing (CYB21191) and the Basic Science and Cutting -edge Technology Research Projects of Chongqing Science and Technology Commission (N.O. cstc2019jcyj- msxmX0203) .