Organoids from patient biopsy samples can predict the response of BC patients to neoadjuvant chemotherapy

作者全名："Shu, Dan; Shen, Meiying; Li, Kang; Han, Xiaojian; Li, Han; Tan, Zhaofu; Wang, Yu; Peng, Yang; Tang, Zhenrong; Qu, Chi; Jin, Aishun; Liu, Shengchun"

作者地址："[Shu, Dan; Shen, Meiying; Li, Kang; Li, Han; Tan, Zhaofu; Wang, Yu; Peng, Yang; Tang, Zhenrong; Qu, Chi; Liu, Shengchun] Chongqing Med Univ, Dept Endocrine & Breast Surg, Affiliated Hosp 1, Chongqing, Peoples R China; [Han, Xiaojian; Jin, Aishun] Chongqing Med Univ, Coll Basic Med, Dept Immunol, Chongqing, Peoples R China; [Han, Xiaojian; Jin, Aishun] Chongqing Med Univ, Chongqing Key Lab Basic & Translat Res Tumor Immu, Chongqing, Peoples R China; [Wang, Yu] Peking Union Med Coll & Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Gen Surg, Beijing, Peoples R China"

通信作者："Liu, SC (通讯作者)，Chongqing Med Univ, Dept Endocrine & Breast Surg, Affiliated Hosp 1, Chongqing, Peoples R China.; Jin, AS (通讯作者)，Chongqing Med Univ, Coll Basic Med, Dept Immunol, Chongqing, Peoples R China.; Jin, AS (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Basic & Translat Res Tumor Immu, Chongqing, Peoples R China."

来源：ANNALS OF MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000863794400001

JCR分区：Q2

影响因子：4.4

年份：2022

卷号：54

期号：1

开始页：2581

结束页：2597

文献类型：Article

关键词：Breast cancer; organoid; biopsy sample; neoadjuvant chemotherapy; complete pathological response; personalized

摘要："Propose Neoadjuvant chemotherapy has been widely used in locally advanced and inflammatory breast cancer. Generally, complete pathological response after neoadjuvant chemotherapy treatment predicts survival. Studies have shown that patient-derived organoids can be used in cancer research and drug development. Therefore, we aimed to generate a living organoid biobank from biopsy samples to predict the response of patients to neoadjuvant chemotherapy. Method We generated a living organoid biobank from locally advanced breast cancer patients receiving neoadjuvant chemotherapy. When the patient received neoadjuvant chemotherapy, the organoids were treated with similar drugs, thereby simulating the situation of the patient receiving treatment. Result We successfully constructed organoids from breast cancer biopsies, demonstrating that organoids can be generated from a small sample of tissue. The phenotype of breast cancer organoid often agreed with the original breast cancer according to the blinded histopathological analysis of H&E stain tissue and organoid sections. In addition, our data confirm that the patient's response to chemotherapy closely matches the organoids' response to drugs. Conclusion Our data indicate that patient-derived organoids can be used to predict the clinical response of breast cancer patients to neoadjuvant chemotherapy in vitro and to screen drugs that have different effects on different patients. Key message Complete pathological response (pCR) after adjuvant chemotherapy can predict, survival, therefore, predicting patient response to neoadjuvant chemotherapy is critical. Patient-derived organoids (PDOs) matched the original tumour in terms of histopathology, hormone receptor levels and HER2 receptor status. Patient-derived organoids can predict the responsiveness of patient to neoadjuvant chemotherapy."

基金机构："National Natural Science Foundation of China (NSFC) [81772979, 81872329]; key research and development project of Chongqing's technology innovation and application development special big health field [CSTC2021jscx-gksb-N0027]; Chongqing Municipal Natural Science Foundation [cstc2018jcyjAX0194]"

基金资助正文：This research was supported by the National Natural Science Foundation of China (NSFC) (Grant number 81772979 and 81872329); the key research and development project of Chongqing's technology innovation and application development special big health field (Project approval number: CSTC2021jscx-gksb-N0027) and Chongqing Municipal Natural Science Foundation (cstc2018jcyjAX0194).