Engrailed homeobox 1 transcriptional regulation of COL22A1 inhibits nasopharyngeal carcinoma cell senescence through the G1/S phase arrest

作者全名："Huang, Mao-Ling; Luo, Wen-Long"

作者地址："[Huang, Mao-Ling; Luo, Wen-Long] Chongqing Med Univ, Dept Otorhinolaryngol Head & Neck Surg, Affiliated Hosp 2, Chongqing, Peoples R China"

通信作者："Luo, WL (通讯作者)，Chongqing Med Univ, Dept Otorhinolaryngol Head & Neck Surg, Affiliated Hosp 2, Chongqing, Peoples R China."

来源：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000863825800001

JCR分区：Q2

影响因子：5.3

年份：2022

卷号：26

期号：21

开始页：5473

结束页：5485

文献类型：Article

关键词：cell cycle; cell senescence; NPC; the CDK4; 6-cyclin D1-Rb signalling pathway

摘要："EN1 is well known as a transcription factor in other tumours, but its role in NPC is unclear. In this study, we first used bioinformatics to analyse GEO data to obtain the differentially expressed gene EN1, and subsequently verified that EN1 was highly expressed in nasopharyngeal carcinoma cells by tissue microarrays as well as cell lines. Further, we down-regulated the expression of EN1 in cells for RNA sequencing. The analysis of sequencing results using KEGG and GO revealed significant changes in cell proliferation and cycle function after downregulation of EN1. Meanwhile, we found that cells underwent senescence after inhibition of EN1 under electron microscopy and the SA-beta-gal assays. Based on the sequencing results, we verified that EN1 can promote the proliferation and cycle of NPC cells in cell function experiments and animal experiments. To investigate how EN1 affects cell senescence, we found that EN1 transcriptional regulation of COL22A1 regulated cell proliferation and cycle via CDK4/6-cyclin D1-Rb signalling pathway by dual luciferase reporter, Immunoblotting and rescue experiment. Accordingly, we uncovered that EN1 could serve as a target for the regulation of senescence in NPC."

基金机构："Chongqing Postdoctoral Special Science Foundation [2010010005500683, 2010010005739004]"

基金资助正文："Chongqing Postdoctoral Special Science Foundation, Grant/Award Number: 2010010005500683 and 2010010005739004"