The intrarenal blood pressure modulation system is differentially altered after renal denervation guided by different intensities of blood pressure responses
作者全名："Lai, Yinchuan; Zhou, Hao; Chen, Weijie; Liu, Hang; Liu, Guangliang; Xu, Yanping; Du, Huaan; Zhang, Bo; Li, Yidan; Woo, Kamsang; Yin, Yuehui"
作者地址："[Lai, Yinchuan; Zhou, Hao; Chen, Weijie; Liu, Hang; Liu, Guangliang; Xu, Yanping; Du, Huaan; Zhang, Bo; Li, Yidan; Woo, Kamsang; Yin, Yuehui] Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 2, Chongqing Cardiac Arrhythmia Therapeut Serv Ctr,C, Chongqing, Peoples R China; [Lai, Yinchuan] Sichuan Univ, Yibin Hosp, Dept Cardiol, Yibin & West China Hosp,Peoples Hosp 2, Yibin City, Sichuan, Peoples R China; [Woo, Kamsang] Chinese Univ Hong Kong, Inst Future Cities, Hong Kong, Peoples R China"
通信作者："Yin, YH (通讯作者)，Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 2, Chongqing Cardiac Arrhythmia Therapeut Serv Ctr,C, Chongqing, Peoples R China."
文献类型：Article; Early Access
关键词：Blood pressure response; Renal denervation; Sympathetic nerve; Renin-angiotensin system; Renal nerve stimulation
摘要："The aim of this study was to investigate alterations in the intrarenal blood pressure (BP) regulation system after renal denervation (RDN) guided by renal nerve stimulation (RNS). Twenty-one dogs were randomized to receive RDN at strong (SRA group, n = 7) or weak (WRA group, n = 7) BP-elevation response sites identified by RNS or underwent RNS only (RNS-control, RSC, n = 7). After 4 weeks of follow-up, renal sympathetic components, the main components of reninangiotensin system (RAS) and the major transporters involved in sodium and water reabsorption were assessed by immunohistochemical analysis. Compared with RSC treatment, RDN therapy significantly reduced renal norepinephrine and tyrosine hydroxylase levels, decreased the renin content and inhibited the onsite generation of angiotensinogen. Moreover, the expression of exciting axis components, including angiotensin-converting enzyme (ACE), angiotensin II and angiotensin II type-1 receptor, was downregulated, while protective axis components for the cardiovascular system, including ACE2 and Mas receptors, were upregulated in both WRA and SRA groups. Moreover, RDN reduced the abundance of aquaporin-1 and aquaporin-2 in kidneys. Although RDN had a minimal effect on overall NKCC2 expression, its activation (p-NKCC2) and directional enrichment in the apical membrane (mNKCC2) were dramatically blunted. All these changes were more obvious in the SRA group than WRA group. Selective RDN guided by RNS effectively reduced systemic BP by affecting the renal neurohormone system, as well as the sodium and water transporter system, and these effects at sites with a strong BP response were more superior."
基金机构：Technology Star Cultivation Program of the Science and Technology Association of Chongqing [KJXX2017017]; Surface project of the Chongqing Municipal Health Bureau [2016MSXM023]; National Natural Science Foundation of China ; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University
基金资助正文："This work was supported, in part, by the Technology Star Cultivation Program of the Science and Technology Association of Chongqing (Grant number: KJXX2017017), the Surface project of the Chongqing Municipal Health Bureau (Grant number: 2016MSXM023), the General Program of the National Natural Science Foundation of China (Grant number: 32071110), and the Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University."