RBM24 Mediates Lymph Node Metastasis and Epithelial-Mesenchymal Transition in Human Hypopharyngeal Squamous Cell Carcinoma by Regulating Twist1

作者全名："Liu, Yuhong; Pan, Min; Lu, Tao; Li, Yanshi; Yu, Dan; Wang, Zhihai; Hu, Guohua"

作者地址："[Liu, Yuhong; Pan, Min; Lu, Tao; Li, Yanshi; Yu, Dan; Wang, Zhihai; Hu, Guohua] Chongqing Med Univ, Affiliated Hosp 1, Dept Otorhinolaryngol, Chongqing 400016, Peoples R China"

通信作者："Hu, GH (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Otorhinolaryngol, Chongqing 400016, Peoples R China."

来源：JOURNAL OF ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000868326700003

JCR分区：Q2

影响因子：4.501

年份：2022

卷号：2022

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Objective. Despite the target RNA regulatory action of RBM24 (RNA Binding Motif 24), a protein implicated in multiple carcinomas, its role in HSCC remains unclear. Our study probed to understand the effect of RBM24 on HSCC. Materials and Methods. A combination of qRT-PCR, IHC, and western blot was employed to assess the HSCC tissue level of RBM24. The colony formation and CCK-8 assays were performed to estimate cellular proliferative potential, whereas the transwell assay was conducted to examine invasive and metastatic potential. The FaDu cell motility was assessed via the scratch-wound assay and EMT (epithelial-mesenchymal transition) by adopting qRT-PCR in conjunction with western blot and IF (immunofluorescence). The in-vivo effect of RBM24 on HSCC was investigated through modeling metastasis to the popliteal LNs (lymph nodes). Results. Among HSCC patients showing metastasis to LNs, prominent RBM24 downregulation was noted, with an intrinsic association between low RBM24 level and poor outcome. Knocking down RBM24 promoted cell multiplication, migration, and infiltration, while overexpression led to the opposite effects and inhibited the EMT. RBM24's suppressive action against the FaDu cell mobility and invasion was reversed by Twist1 overexpression. RBM24's suppressive actions against the tumor evolution and LN metastasis in HSCC in-vivo were also validated. Conclusion. As a carcinoma inhibitor gene, RBM24 regulates Twist1 to achieve LN metastasis and EMT suppression in HSCC."

基金机构：National Natural Science Foundation of China; Natural Science Foundation of Chongqing; [81902776]; [cstc2019jcyj-msxm0325]; [2020MSXM091]

基金资助正文："AcknowledgmentsThis work was supported by the National Natural Science Foundation of China (No. 81902776) and the Natural Science Foundation of Chongqing (cstc2019jcyj-msxm0325, 2020MSXM091)."