Effects of Camk2b Overexpression and Underexpression on the Proteome of Rat Hippocampal Neurons

作者全名："Yang, Chen; Zhang, Mingming; Li, Shuiming; Yi, Faping; Huang, Haojun; Xie, Hong; Liu, Hangfei; Huang, Rongzhong; Zhou, Jian"

作者地址："[Yang, Chen; Zhang, Mingming; Yi, Faping; Huang, Haojun; Zhou, Jian] Chongqing Med Univ, Basic Med Coll, Inst Neurosci, Chongqing 400016, Peoples R China; [Li, Shuiming] Shenzhen Univ, Shenzhen Key Lab Microbiol & Gene Engn, Shenzhen 518060, Peoples R China; [Xie, Hong] Fifth Peoples Hosp Chongqing, Dept Pharm, 24 Renji Rd, Chongqing 400062, Peoples R China; [Liu, Hangfei] Shenzhen Wininnovate Biotech Co Ltd, Shenzhen 518073, Peoples R China; [Huang, Rongzhong] Chongqing Inst Life Sci, Stat Lab, Chongqing 400016, Peoples R China"

通信作者："Xie, H (通讯作者)，Fifth Peoples Hosp Chongqing, Dept Pharm, 24 Renji Rd, Chongqing 400062, Peoples R China.; Zhou, J (通讯作者)，Chongqing Med Univ, Inst Neurosci, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：NEUROSCIENCE

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000870517100005

JCR分区：Q3

影响因子：3.3

年份：2022

卷号：503

期号：　

开始页：58

结束页：68

文献类型：Article

关键词：Camk2b; hippocampal neuron; iTRAQ; Mapk3; proteomics

摘要："studies have demonstrated that Camk2b expression is modified in neuropsychiatric illnesses and potentially affects synaptic plasticity. However, the molecular events arising from Camk2b dysregulation are not fully elucidated and need to be comprehensively explored. In the present study, we first induced over -expression and under-expression of Camk2b in cultured rat hippocampal neurons through transfection with len-tivirus plasmids. Then isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomics followed by bioinformatics analyses were carried out to explore the impacts of Camk2b dysexpression on the pro-teome of the neurons. Compared with the respective controls, a total of 270 proteins in the Camk2b-overexpression group and 209 proteins in the Camk2b-underexpression group were experienced a divergence in expression. Gene ontology and pathway analyses indicated that Camk2b overexpression and under -expression respectively induced two different change profiles of protein expressions and functions, reflecting the potential differences in cellular processes and biological events. Through cross comparison, several candi-date target proteins regulated directly by Camk2b were revealed. Further network and immunoblot analyses demonstrated that Mapk3 could be an important linker and Camk2b-Mapk3 might serve as a new potential path-way affecting the expression of synaptic proteins in hippocampal neurons. Collectively, the present results offer a new comprehension of the regulatory molecular mechanism of Camk2b and thereby increase our understanding of Camk2b-mediated synaptogenesis in synaptic plasticity.(c) 2022 IBRO. Published by Elsevier Ltd. All rights reserved."

基金机构：National Natural Science Foundation of China; Health and Family Planning Commission of Chongqing Nanan District; [31770890]; [31570826]; [2018-03]

基金资助正文："This work was supported by the National Natural Science Foundation of China (Grants No. 31770890, 31570826) and the Health and Family Planning Commission of Chongqing Nanan District (Grant No. 2018-03) ."