Inhibition of miR-423-5p Exerts Neuroprotective Effects in an Experimental Rat Model of Cerebral Ischemia/Reperfusion Injury

作者全名："Luo, Jing; Jiang, Ning; Chen, Jialei; Yu, Gao; Zhao, Jing; Yang, Changhong; Zhao, Yong"

作者地址："[Luo, Jing; Jiang, Ning; Yu, Gao; Yang, Changhong; Zhao, Yong] Chongqing Med Univ, Dept Pathol, Chongqing 400016, Peoples R China; [Chen, Jialei] Chongqing Med Univ, Dept Otorhinolaryngol, Affiliated Hosp 1, Chongqing, Peoples R China; [Zhao, Jing] Chongqing Med Univ, Dept Pathophysiol, Chongqing 400016, Peoples R China; [Yang, Changhong] Chongqing Med Univ, Dept Bioinformat, Chongqing 400016, Peoples R China; [Luo, Jing; Jiang, Ning; Yu, Gao; Zhao, Yong] Chongqing Med Univ, Dept Pathol, Affiliated Hosp 1, Chongqing 400016, Peoples R China"

通信作者："Yang, CH; Zhao, Y (通讯作者)，Chongqing Med Univ, Dept Pathol, Chongqing 400016, Peoples R China."

来源：NEUROSCIENCE

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000870517100008

JCR分区：Q3

影响因子：3.3

年份：2022

卷号：503

期号：　

开始页：95

结束页：106

文献类型：Article

关键词：cerebral I; R injury; miR-423-5p; NLRP3 inflammasome; apoptosis; oxidative stress

摘要："(miRNAs) are widely acknowledged to play a unique role in cerebrovascular disease. This research investigates the function of microRNAs in ischemic stroke via a middle cerebral artery occlusion (MCAO) model. Four differentially expressed microRNAs in rat brains were identified by bioinformatics analysis, and qRT-PCR showed that miR-423-5p exhibited the highest expression in cerebral ischemia/reperfusion injury in rats, with peak levels observed at 24 hours. After microRNA inhibitors and mimics were administrated in the rat model of MCAO, the neurological scores and brain water content were detected, and triphenyltetrazolium chloride (TTC), Hematoxylin and Eosin (H&E), and Nissl staining were conducted to explore the influence of miR-423-5p on ischemic stroke. Subsequently, western blot, ELISA, MPO, TUNEL and commercial assay kits were applied to assess the influence of miR-423-5p on NLRP3 inflammasome, apoptosis, and oxidative stress levels in ischemic penumbra tissue. The results showed that miR-423-5p knockdown could effectively improve neurological indica-tors, such as cerebral infarct volume, brain water content, neurological scores, and nerve tissue damage, and inhibit the NLRP3 inflammasome, apoptosis, and oxidative stress. In contrast, the miR-423-5p mimic yielded opposite results. In conclusion, inhibition of miR-423-5p expression could effectively attenuate ischemic stroke and might be considered a promising target for stroke.(c) 2022 IBRO. Published by Elsevier Ltd. All rights reserved."

基金机构：Natural Science Foundation of Chongqing; National Natural Science Foundation of China [81771261]

基金资助正文："This study was supported by the Natural Science Foundation of Chongqing (No.cstc2020jcyj-bshX0069, No.cstc2020jcyj-msxmX0144) and the National Natural Science Foundation of China (No. 81771261)."