"Effect of Interleukin 6 on Scleral Fibroblast Proliferation, Differentiation, and Apoptosis Involved in Myopic Scleral Remodeling"

作者全名："Liu, Ling; Zhou, Wenjun; Fan, Yujie; Zhang, Liming; Liu, Shichun; Song, Shengfang; Li, Hua"

作者地址："[Liu, Ling; Zhou, Wenjun; Fan, Yujie; Zhang, Liming; Liu, Shichun; Song, Shengfang; Li, Hua] Chongqing Med Univ, Yongchuan Hosp, Dept Ophthalmol, Chongqing, Peoples R China"

通信作者："Liu, L (通讯作者)，Chongqing Med Univ, Yongchuan Hosp, Dept Ophthalmol, Chongqing, Peoples R China."

来源：OPHTHALMIC RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000875571800005

JCR分区：Q2

影响因子：2.1

年份：2022

卷号：65

期号：5

开始页：529

结束页：539

文献类型：Article

关键词：Myopia; Hypoxia; Interleukin 6; Scleral fibroblasts; Scleral remodeling

摘要："Introduction: Scleral hypoxia (HO) is present in myopic eyes, and interleukin (IL)-6 is increased in the aqueous humor of patients with high myopia. The aim of this study was to investigate the effects of IL-6 on scleral fibroblast proliferation, differentiation, and apoptosis under conditions of HO and the possible role of IL-6 in myopic scleral remodeling. Methods: Primary human scleral fibroblasts (HSFs) were cultured using a tissue mass adherent method. First, cells were cultured under conditions of HO (2% O-2) or normoxia (NO, 20% O-2) for different times. A quantitative real-time polymerase chain reaction (qRT-PCR) and immunofluorescence were used to detect the expression of IL-6 in HSFs. Next, cells were divided into five groups: NO, HO, HO plus IL-6, HO plus IL-6 receptor inhibitor (IL6RI), and HO plus IL-6 and IL6RI. The groups were treated separately for 72 h. Cell counting kit-8 assay and flow cytometry were used to detect cell proliferation and apoptosis, respectively. Western blotting and qRT-PCR were used to detect the expression of various genes in the transforming growth factor-beta 1/Smad2/matrix metalloproteinase-2 pathway; these methods and immunofluorescence were also used to detect transdifferentiation of HSFs. Results: HO resulted in upregulation of IL-6 expression in HSFs. Compared with NO, HO resulted in diminished cell proliferation and increased apoptosis and differentiation in HSFs; the above trend was further enhanced by the addition of IL6RI. Compared with the HO group, the addition of IL-6 led to a decrease in cell proliferation and an increase in apoptosis and differentiation of HSFs; the above trends showed opposite changes after the addition of both IL-6 and IL6RI. Additionally, IL-6 and IL6RI exerted opposite regulatory effects on the transforming growth factor-beta 1/Smad2/matrix metalloproteinase-2 pathway under conditions of HO. Conclusion: HO caused HSFs to overexpress IL-6. IL-6 has a role in scleral remodeling in myopic eyes through affecting the proliferation, differentiation, and apoptosis of HSFs."

基金机构："Chongqing Social Enterprise and Livelihood Security Special General Project; Research Project of the Postgraduate Innovation Fund of Yongchuan Hospital, Chongqing Medical University; [cstc2016shmszx130070]; [YJSCX202007]"

基金资助正文："This work was supported by the Chongqing Social Enterprise and Livelihood Security Special General Project (No. cstc2016shmszx130070) and Research Project of the Postgraduate Innovation Fund of Yongchuan Hospital, Chongqing Medical University (No. YJSCX202007). These sources provided support for all reagents and consumables in this research."