DNA methylation alternation in Stanford- A acute aortic dissection

作者全名："Chen, Yufei; Xu, Xu; Chen, Zhaoran; Huang, Bi; Wang, Xiaojian; Fan, Xiaohan"

作者地址："[Chen, Yufei; Xu, Xu; Chen, Zhaoran; Huang, Bi; Fan, Xiaohan] Chinese Acad Med Sci, Fuwai Hosp, Peking Union Med Coll, Natl Ctr Cardiovasc Dis,Dept Cardiol, Beijing, Peoples R China; [Chen, Zhaoran] Capital Med Univ, Beijing Friendship Hosp, Dept Geriatr & Gerontol, Beijing, Peoples R China; [Huang, Bi] Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 1, Chongqing, Peoples R China; [Wang, Xiaojian] Chinese Acad Med Sci & Peking Union Med Coll, Fuwai Hosp, Natl Ctr Cardiovasc Dis, Key Lab Pulm Vasc Med,State Key Lab Cardiovasc Di, Beijing, Peoples R China"

通信作者："Fan, XH (通讯作者)，Chinese Acad Med Sci, Fuwai Hosp, Peking Union Med Coll, Natl Ctr Cardiovasc Dis,Dept Cardiol, Beijing, Peoples R China.; Wang, XJ (通讯作者)，Chinese Acad Med Sci & Peking Union Med Coll, Fuwai Hosp, Natl Ctr Cardiovasc Dis, Key Lab Pulm Vasc Med,State Key Lab Cardiovasc Di, Beijing, Peoples R China."

来源：BMC CARDIOVASCULAR DISORDERS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000876574800004

JCR分区：Q3

影响因子：2.1

年份：2022

卷号：22

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：DNA methylation; Epigenetics; Acute aortic dissection; Vascular

摘要："Background Acute aortic dissection (AAD) is a life-threatening cardiovascular disease. Recent studies have shown that DNA methylation may be associated with the pathological mechanism of AAD, but the panorama of DNA methylation needs to be explored. Methods DNA methylation patterns were screened using Infinium Human Methylation 450 K BeadChip in the aortic tissues from 4 patients with Stanford-A AAD and 4 controls. Gene enrichment was analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and gene ontology (GO). DNA methylation levels of candidate genes were determined by pyrosequencing in the replication cohort including 16 patients with AAD and 7 controls. Protein expression level of candidate gene was assessed by Western blot. Results A total of 589 differentially methylated positions including 315 hypomethylated and 274 hypermethylated positions were found in AAD group. KEGG analysis demonstrated that differentially methylated position-associated genes were enriched in MAPK signaling pathway, TNF signaling pathway and apoptosis pathway, et al. GO analysis demonstrated that differentially methylated position-associated genes were enriched in protein binding, angiogenesis and heart development et al. The differential DNA methylation in five key genes, including Fas, ANGPT2, DUSP6, FARP1 and CARD6, was authenticated in the independent replication cohort. The protein expression level of the Fas was increased by 1.78 times, indicating the possible role of DNA methylation in regulation of gene expression. Conclusion DNA methylation was markedly changed in the aortic tissues of Stanford-A AAD and associated with gene dysregulation, involved in AAD progression."

基金机构：Beijing Natural Science Foundation [J200008]

基金资助正文：This work was supported by supported by Beijing Natural Science Foundation (J200008).