The Relationship Between Klotho and SIRT1 in Renal Related Disease

作者全名："Su, Hong; Gao, Diansa; Chen, Yanlin; Zuo, Zhong"

作者地址："[Su, Hong; Gao, Diansa; Chen, Yanlin; Zuo, Zhong] Chongqing Med Univ, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Su, Hong] Chongqing Key Lab Translat Med Major Metab Dis, Chongqing 400016, Peoples R China; [Su, Hong] Shenyang KingMed Ctr Clin Lab Co Ltd, Dept Pathol, Shenyang 110164, Peoples R China"

通信作者："Zuo, Z (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Chongqing 400016, Peoples R China."

来源：INTERNATIONAL JOURNAL OF GENERAL MEDICINE

ESI学科分类：　

WOS号：WOS:000876768400001

JCR分区：Q2

影响因子：2.3

年份：2022

卷号：15

期号：　

开始页：7885

结束页：7893

文献类型：Article

关键词：renal arteriosclerosis; aging; Klotho; SIRT1

摘要："Background: This study focused on renal arteriosclerosis and aimed to explore the relationship between Klotho and SIRT1 by morphological staining, which will help to provide new ideas for the treatment of renal-aging-related diseases and a theoretical basis for the development of new drugs. Methods: Kidney tissue samples were collected from patients who underwent nephrectomy. HK-2 cells were cultured. The Hematoxylin-eosin (HE) staining, Periodic Acid-Schiff (PAS) staining, Masson's Trichrome staining, Immunohistochemistry (IHC) staining, Immunofl uorescence (ICC) and bioinformatics means were used for this study. Results: HE staining showed that glomerulosclerosis was atrophic and cast was significantly increased luminal narrowing of renal arterioles in aging group. PAS staining showed that the number of podocytes was reduced, the mesangial matrix expansion and the intimal fibrosis of renal arterioles. Masson's trichrome staining showed that there was massive collagen proliferation in the tubulointerstitial in aging group, as well as intimal thickening and fibrin deposition in the tubular walls of arterioles. IHC staining showed that the expression of Klotho and SIRT1 protein was downregulated in aging group and the trend of the two was positively correlated (P < 0.01). Klotho and SIRT1 co-localized in HK-2 cells and kidney tissue. The GEPIA database analysis showed a significant positive correlation between Klotho and SIRT1 in multiple human tissues and tumors. Conclusion: Glomerulosclerosis in aging group is accompanied by low expression of Klotho and SIRT1 in renal tissue, and Klotho is positively correlated with SIRT1. Klotho-SIRT1 pathway may be involved in the occurrence and development of renal-aging-related diseases."

基金机构：National Nature Science Foundation of China; Fundamental Science and Advanced Technology Research of Chongqing; [81701384]; [CSTC2019jcyjmsxm0425]

基金资助正文：Funding This work was supported by the National Nature Science Foundation of China (No. 81701384) and the Fundamental Science and Advanced Technology Research of Chongqing (CSTC2019jcyjmsxm0425) .