Comparison of the biomechanical differences in the occlusal movement of wild-type and BMP9 knockout mice with apical periodontitis

作者全名："Peng, Mengtian; Ma, Xiaofei; Cao, Yuying; Wang, Chunjuan; Tan, Qin; Chu, Xinyue; Zhou, Pengfei; Fu, Tiwei; Liang, Panpan; Ran, Shidian; He, Tong-Chuan; Wang, Jinhua; Huang, Enyi"

作者地址："[Peng, Mengtian; Ma, Xiaofei; Wang, Chunjuan; Tan, Qin; Chu, Xinyue; Zhou, Pengfei; Fu, Tiwei; Liang, Panpan; Ran, Shidian; Wang, Jinhua; Huang, Enyi] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing, Peoples R China; [Peng, Mengtian; Ma, Xiaofei; Wang, Chunjuan; Tan, Qin; Chu, Xinyue; Zhou, Pengfei; Fu, Tiwei; Liang, Panpan; Ran, Shidian; Wang, Jinhua; Huang, Enyi] Chongqing Med Univ, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Coll Stomatol, Chongqing, Peoples R China; [Cao, Yuying] Chongqing Med Univ, Dept Stomatol, Childrens Hosp, Chongqing, Peoples R China; [He, Tong-Chuan] Univ Chicago Med Ctr, Dept Orthopaed Surg & Rehabil Med, Mol Oncol Lab, Chicago, IL USA"

通信作者："Wang, JH; Huang, EY (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing, Peoples R China.; Wang, JH; Huang, EY (通讯作者)，Chongqing Med Univ, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Coll Stomatol, Chongqing, Peoples R China."

来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

ESI学科分类：Multidisciplinary

WOS号：WOS:000878091700001

JCR分区：Q1

影响因子：5.7

年份：2022

卷号：10

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：apical periodontitis; occlusal movement; BMP9; biomechanics; finite element analysis

摘要："Apical periodontitis is a common clinical disease caused by bacteria; bacterial metabolites can cause an imbalance in bone homeostasis, bone mass reduction, and tooth loss. Bone resorption in apical periodontitis causes a concentration of stress in the tooth and periodontal tissues during occlusion, which aggravates the disease. Emerging evidence indicates that bone morphogenetic protein 9 (BMP9), also known as growth differentiation factor 2(Gdf2), may play an important role in tooth and dentoalveolar development. Herein, we investigated the role of BMP9 in the development of apical periodontitis and its effects on the biomechanics of dentoalveolar bone. Apical periodontitis models were established in five BMP9 knockout (KO) mice and five C57BL/6 WT (wild-type) mice. At baseline and 14, 28, and 42 days after modeling, in vivo micro-computed tomography analysis and three-dimensional (3D) reconstruction were performed to evaluate the apical lesion in each mouse, and confirm that the animal models were successfully established. Finite element analysis (FEA) was performed to study the stress and strain at the alveolar fossa of each mouse under the same vertical and lateral stress. FEA revealed that the stress and strain at the alveolar fossa of each mouse gradually concentrated on the tooth cervix. The stress and strain at the tooth cervix gradually increased with time but were decreased at day 42. Under the same lingual loading, the maximum differences of the stress and strain at the tooth root in KO mice were greater than those in WT mice. Thus, these findings demonstrate that BMP9 could affect the biomechanical response of the alveolar fossa at the tooth root in mice with apical periodontitis. Moreover, the effects of BMP9 on the biomechanical response of the alveolar bone may be site-dependent. Overall, this work contributes to an improved understanding of the pathogenesis of apical periodontitis and may inform the development of new treatment strategies for apical periodontitis."

基金机构："National Natural Science Foundation of China [cstc2018jcyjAX0731]; Natural Science Foundation of Chongqing, China [KJQN202100437]; Scientific and Technological Research Program of Chongqing Municipal Education Commission; Scientific and Technological Research Program of Chongqing Yubei district; [81301551]; [cstc2020jcyjmsxmX0380]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (81301551), the Natural Science Foundation of Chongqing, China (cstc2020jcyjmsxmX0380 and cstc2018jcyjAX0731), the Scientific and Technological Research Program of Chongqing Municipal Education Commission (KJQN202100437), and the Scientific and Technological Research Program of Chongqing Yubei district (2021 nongshe 20)."