Efficacy of Probiotic Consortium Transplantation on Experimental Necrotizing Enterocolitis

作者全名："Tian, Bing; Zhang, Yunfei; Deng, Chun; Guo, Chunbao"

作者地址："[Zhang, Yunfei; Deng, Chun] Chongqing Med Univ, Childrens Hosp, Key Lab Child Dev & Disorders, Minist Educ, Chongqing, Peoples R China; [Deng, Chun] Chongqing Med Univ, Yongchuan Hosp, Dept Pediat, Chongqin, Peoples R China; [Zhang, Yunfei; Deng, Chun; Guo, Chunbao] Chongqing Univ Technol, Sch Pharm & Bioengn, Chongqing, Peoples R China; [Zhang, Yunfei] Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders Chongqing, Chongqing, Peoples R China; [Zhang, Yunfei; Deng, Chun; Guo, Chunbao] Chongqing Med Univ, Childrens Hosp, China Int Sci & Technol Cooperat Base Child Dev &, Chongqing, Peoples R China; [Deng, Chun; Guo, Chunbao] Chongqing Med Univ, Childrens Hosp, Chongqing Key Lab Pediat, Chongqing, Peoples R China; [Guo, Chunbao] Chongqing Med Univ, Women & Chidrens Hosp, Dept Pediat Surg, Chongqing, Peoples R China; [Deng, Chun] Chongqing Med Univ, Yongchuan Hosp, Dept Pediat, 439 Xuanhua Rd, Chongqing 402160, Peoples R China; [Guo, Chunbao] Chongqing Med Univ, Women & Childrens Hosp, Dept Pediat Surg, 120 Longshan Rd, Chongqing 400014, Peoples R China"

通信作者："Deng, C (通讯作者)，Chongqing Med Univ, Yongchuan Hosp, Dept Pediat, 439 Xuanhua Rd, Chongqing 402160, Peoples R China.; Guo, CB (通讯作者)，Chongqing Med Univ, Women & Childrens Hosp, Dept Pediat Surg, 120 Longshan Rd, Chongqing 400014, Peoples R China."

来源：JOURNAL OF SURGICAL RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000878327500008

JCR分区：Q2

影响因子：2.2

年份：2022

卷号：279

期号：　

开始页：598

结束页：610

文献类型：Article

关键词：Bacteria transplantation; Necrotizing enterocolitis; Oxidative stress

摘要："Introduction: Fecal microbiota transplantation (FMT) is a promising therapy, but it has not been used to treat neonatal necrotizing enterocolitis (NEC) due to reports of adverse side effects. Probiotics are considered relatively safe with practicable administrative proced-ures; however, no systematic research has compared the results of FMT and probiotic consortium transplantation (PCT) on oxidative stress in the intestines of patients with NEC. We conducted this study to provide a basis for optimizing NEC therapy.Methods: Eight-day-old newborn C57BL/6 mice were randomly divided into the following four groups: the dam-fed group (control group); the NEC induction group (NEC group); the NEC induction and transplantation of Lactobacillus reuteri and Bifidobacterium infantis con-sortium group (NEC + PCT group); and the NEC induction and the FMT group (NEC + FMT). Intestinal injury, oxidative stress indexes, intestinal barrier function, and inflammatory cytokines were assessed in the terminal ileum.Results: FMT more effectively modulates oxidative stress in the intestine than does PCT; however, the difference between the effects of PCT and FMT was not significant. The protective effect was associated with enhanced antioxidant capacity, regulation of the main components of the mucus layer, reduced inflammatory reactions, and improved intestinal integrity.Conclusions: Intestinal dysbiosis affects oxidative stress, inflammatory response, and mucosal integrity. Although FMT is more effective than PCT in regulating oxidative stress, PCT may be preferred in pediatrics because the proportion and dose of transplanted bac-teria can be standardized and individualized according to individual conditions.(c) 2022 Published by Elsevier Inc."

基金机构："National Natural Science Foundation of China, China [30973440, 30770950]; Chongqing Natural Science Foundation, China (CSTC) [2008BA0021, cstc2012jjA0155]"

基金资助正文："y The research was supported by the National Natural Science Foundation of China, China (No: 30973440, 30770950) , and the key project of the Chongqing Natural Science Foundation, China (CSTC, 2008BA0021, cstc2012jjA0155) ."