Self-propelled Janus nanomotor as active probe for detection of pepsinogen by lateral flow immunoassay
作者全名："Guo, Jiuchuan; Li, Yang; Wang, Bo; Chen, Wenjun; Chen, Shuqin; Liu, Shan; Ma, Xing; Guo, Jinhong"
作者地址："[Guo, Jiuchuan; Guo, Jinhong] Chongqing Med Univ, Coll Lab Med, MOE Key Lab Lab Med Diagnost, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China; [Guo, Jiuchuan] Univ Elect Sci & Technol China, Sch Automat Engn, Chengdu 611731, Peoples R China; [Li, Yang; Wang, Bo] Shanghai Univ Engn Sci, Sch Mat Engn, Shanghai 201620, Peoples R China; [Chen, Wenjun] Harbin Inst Technol Shenzhen, Sch Mat Sci & Engn, Sauvage Lab Smart Mat, Shenzhen 518055, Peoples R China; [Chen, Shuqin; Ma, Xing] Harbin Inst Technol Shenzhen, Sch Mat Sci & Engn, Shenzhen 518055, Peoples R China; [Liu, Shan] Univ Elect Sci & Technol, Sichuan Acad Med Sci, Dept Lab Med, Chengdu 610072, Peoples R China; [Liu, Shan] Univ Elect Sci & Technol, Sichuan Prov Peoples Hosp, Chengdu 610072, Peoples R China"
通信作者："Ma, X (通讯作者)，Harbin Inst Technol Shenzhen, Sch Mat Sci & Engn, Shenzhen 518055, Peoples R China."
关键词：Janus nanomotor; Active mode; Lateral flow immunoassay; Pepsinogen quantification; Point-of-care testing; Test strip; ImageJ evaluation
摘要："The feasibility of using nanomotors as active probes for lateral flow immunoassay (LFIA) is demonstrated. We synthesized Au@mSiO(2)@Pt Janus nanomotor, where nanolayer of Pt was deposited on the half side surface of the Au@mSiO(2) nanoparticles, which can catalyze the decomposition of H2O2 to produce driving force for the nanomotor. Subsequently, the motion characteristics of the Au@mSiO(2)@Pt nanomotor in static fluidic environment and dynamic flow field was studied to pave the way for its practical application in lateral flow immunoassay (LFIA). At last, the Au@mSiO(2)@Pt nanomotor was modified with antibody and then used as active immunoassay probe in LFIA. We chose gastric function index, pepsinogen II (PG II) and pepsinogen II (PG II), as the target analytes. The results indicated that, compared with traditional Au nanoprobe, the nanomotor-based probe can significantly improve the sensitivity by increasing the probability and efficiency of antigen and antibody binding. A limit of detection (LOD) of 2.2 ng/mL for PGI, and 2.1 ng/mL for PG II was achieved. This work provides a new solution for enhancing the capability of immune detection, and we believe the nanomotor-based LFIA will have great potential in high-sensitivity point-of-care-testing in the future."
基金机构："National Natural Science Foundation of China [62122017, 52072095, 92163109]; Shenzhen Science and Technology Program [JCYJ20200109113408066, KQTD20170809110344233]; Fundamental Research Funds for the Central Universities [HIT. OCEF. 2021032]; National Science Fund for Excellent Young Scholars ; Shenzhen Bay Laboratory [SZBL2019062801005]"
基金资助正文："The authors thank the financial support from National Natural Science Foundation of China (62122017, 52072095, 92163109), the Shenzhen Science and Technology Program (JCYJ20200109113408066, KQTD20170809110344233), Shenzhen Bay Laboratory (SZBL2019062801005), the Fundamental Research Funds for the Central Universities (Grant No. HIT. OCEF. 2021032), the National Science Fund for Excellent Young Scholars (6212200344)."