Polydopamine assembled stable core-shell nanoworms-DNAzyme probe for selective detection of Pb2+and in living cells imaging

作者全名："Yu, Yujie; Qing, Min; Ma, Yidan; Zhang, Yan; Zheng, Shuting; Yu, Chao; He, Junlin"

作者地址："[Yu, Yujie; Ma, Yidan; Zhang, Yan; Zheng, Shuting; He, Junlin] Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China; [Yu, Yujie; Ma, Yidan; Zhang, Yan; Zheng, Shuting; He, Junlin] Chongqing Med Univ, Joint Int Res Lab Reprod & Dev, Chongqing 400016, Peoples R China; [Qing, Min; Yu, Chao] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China"

通信作者："He, JL (通讯作者)，Chongqing Med Univ, Sch Publ Hlth & Management, Chongqing 400016, Peoples R China."

来源：TALANTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000888088300004

JCR分区：Q1

影响因子：6.1

年份：2023

卷号：253

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Lead ion; Pb2+-specific DNAzyme; Cell imaging; Polydopamine; Nanoworms

摘要："Lead ion (Pb2+) is a major non-degradable environmental pollutant and poses threat to human health. DNAzymes have proved to be a promising tool for the detection of extracellular metal ions. Owing to cellular uptake, enzymatic degradation and reliable signal output, using DNAzymes to quantitative intracellular sensing of Pb2+ still presents challenges. In this study, we report a core-shell nanoworms-DNAzyme nanoprobe combing hairpin -like DNAzyme (H DNAzyme) with polydopamine-coated Au cores plasmonic ""nanoworms"" (Au@PDA NWs) for imaging of Pb2+ in living cells. PDA with good biocompatibility, high near-infrared (NIR) fluorescence quenching efficiency, and provides a larger surface for further functionalization of biomolecules. H DNAzyme can loaded onto Au@PDA NWs via coordination interaction and 7C-7C stacking, resulting in significant quenching of their fluorescence by PDA shells and Au cores. In the presence of target Pb2+, the H DNAzyme are activated and release Cy5 fluorophore-labeled short strands from Au@PDA NWs. Therefore, an increased fluorescence signal is observed and allows imaging of Pb2+ in living cells. Moreover, the highly selective of H DNAzyme make the proposed method effectively discriminate against other nonspecific molecules. The Au@PDA NWs-H DNAzyme nanoprobe shows good biocompatibility and obviously decreased background fluorescence, which can monitor Pb2+ levels and biodistribution. The developed nanoprobe holds great potential for further extend to bioimaging of other metal ions."

基金机构：Construction Project of Postgraduates Tutor Team of Chongqing; [dstd201809]

基金资助正文：This work was supported by grants from Construction Project of Postgraduates Tutor Team of Chongqing (Grant no. dstd201809) .