A novel binary luminophore based high-efficient electrochemiluminescence biosensor for ultrasensitive detection of human epidermal growth factor receptor-2

作者全名："Xie, Jiaojiao; Mu, Zhaode; Qing, Min; Zhou, Jing; Sun, Shaochen; Bai, Lijuan"

作者地址："[Xie, Jiaojiao; Zhou, Jing; Sun, Shaochen; Bai, Lijuan] Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China; [Mu, Zhaode; Qing, Min] Chongqing Med Univ, Coll Pharm, Res Ctr Pharmacodynam Evaluat Engn Technol Chongq, Chongqing 400016, Peoples R China"

通信作者："Bai, LJ (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Res Ctr Pharmaceut Engn, Chongqing 400016, Peoples R China."

来源：CHEMICAL ENGINEERING JOURNAL

ESI学科分类：ENGINEERING

WOS号：WOS:000888228300001

JCR分区：Q1

影响因子：15.1

年份：2022

卷号：450

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：ECL biosensor; Binary luminophore; Boron carbon oxynitride; Breast cancer

摘要："For the first time, a high-efficient and low-cost electrochemiluminescence (ECL) biosensor was constructed for ultrasensitive detection of breast cancer related human epidermal growth factor receptor-2 (HER-2) using a novel binary luminophore and low concentration of coreactant. Firstly, Au nanoparticles decorated graphite-like carbon nitride (g-C3N4@Au) was designed as sensing platform, which can generate ECL signal in the potential range from -1.2 V to 0 V when peroxydisulfate (S2O82-) was used as the coreactant. Secondly, amino-modified capture probes (CP) were immobilized on the surface of g-C3N4@Au via Au-N bonds. In addition, Zr-based metal organic framework (Zr-MOF) modified bulk boron carbon oxynitride (BCNO), named ZMB, exhibited remarkable ECL intensity at the potential window of -2.0 V to 0 V with 0.8 mM S2O82-. ZMB possessed a large specific surface area and abundant amino groups to anchor Au@Pt nanoparticles (Au@PtNPs). Therefore, HER-2 binding aptamers (HBA) could be anchored on Au@PtNPs to form the tracer label. Under optimal conditions, the proposed ECL biosensor displayed a wide linear range from 10 fg mL(-1) to 100 ng mL(-1) with a limit of detection (LOD) as low as 0.2 fg mL(-1). Importantly, this work utilized ZMB/S2O82- as new ECL system with satisfactory detection performances, which provided a potential approach to improve the sensitivity in clinical diagnosis."

基金机构："National Natural Science Foundation of China [82072378, 81601856]; Chongqing Talents: Exceptional Young Talents Project, China [CQYC202005015, cstc2021ycjh-bgzxm0328]; Ba Yu Scholar Program, China [YS2019020]; Program for Youth Innovation in Future Medicine, Chongqing Medical University, China [W0067]; Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China; Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China [YXY2019XSGG4, YXY2021BSH04]; Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City, China [cstc2014kjrc-qnrc00004]"

基金资助正文："This work is supported by National Natural Science Foundation of China (82072378, 81601856), Chongqing Talents: Exceptional Young Talents Project, China (CQYC202005015, cstc2021ycjh-bgzxm0328), Ba Yu Scholar Program, China (YS2019020), Program for Youth Innovation in Future Medicine, Chongqing Medical University, China (W0067), Funds for High Level Young Science and Technology Talent Cultivation Plan in Chongqing Medical University, China (2019), Discipline Talent Training Program of College of Pharmacy in Chongqing Medical University, China (YXY2019XSGG4, YXY2021BSH04) and Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City, China (cstc2014kjrc-qnrc00004)."