Research of childhood tuberculosis in suspected populations by molecular methods: A multicenter study in China

作者全名："Li, Chunling; Wang, Shifu; Yu, Hui; Wang, Jiangxia; Deng, Jikui; Wang, Hongmei; Hua, Chunzhen; Zhuo, Zhiqiang; Chen, Lei; Hao, Jianhua; Gao, Wei; Zhang, Hong; Zhang, Ting; Xu, Hongmei; Wang, Chuanqing"

作者地址："[Li, Chunling; Wang, Chuanqing] Fudan Univ, Childrens Hosp, Childrens Natl Med Ctr, Clin Microbiol Lab, Shanghai, Peoples R China; [Wang, Shifu] Shandong Univ, Childrens Hosp, Shandong Prov Clin Res Ctr Childrens Hlth & Dis, Jinan, Shandong, Peoples R China; [Yu, Hui] Fudan Univ, Childrens Hosp, Childrens Natl Med Ctr, Div Infect Dis, Shanghai, Peoples R China; [Wang, Jiangxia; Xu, Hongmei] Chongqing Med Univ, Childrens Hosp, Dept Infect Dis, Chongqing, Peoples R China; [Deng, Jikui; Wang, Hongmei] Shenzhen Childrens Hosp, Dept Infect Dis, Shenzhen, Guangdong, Peoples R China; [Hua, Chunzhen] Zhejiang Univ, Sch Med, Childrens Hosp, Div Infect Dis, Hangzhou, Zhejiang, Peoples R China; [Zhuo, Zhiqiang] Fudan Univ, Childrens Hosp, Xiamen Branch, Xiamen Childrens Hosp,Dept Infect Dis, Xiamen, Fujian, Peoples R China; [Chen, Lei] Fudan Univ, Childrens Hosp, Xiamen Childrens Hosp, Xiamen Branch,Dept Med Lab Diag Ctr, Xiamen, Fujian, Peoples R China; [Hao, Jianhua] Childrens Hosp Kaifeng City, Dept Med Lab Diag Ctr, Kaifeng, Henan, Peoples R China; [Gao, Wei] Childrens Hosp Kaifeng City, Dept Infect Dis, Kaifeng, Henan, Peoples R China; [Zhang, Hong] Shanghai Jiao Tong Univ, Childrens Hosp, Sch Med, Dept Med Lab Diag Ctr, Shanghai, Peoples R China; [Zhang, Ting] Shanghai Jiao Tong Univ, Sch Med, Shanghai Childrens Hosp, Institue Pediat Infect Immun & Crit Care Med, Shanghai, Peoples R China"

通信作者："Wang, CQ (通讯作者)，Fudan Univ, Childrens Hosp, Childrens Natl Med Ctr, Clin Microbiol Lab, Shanghai, Peoples R China.; Xu, HM (通讯作者)，Chongqing Med Univ, Childrens Hosp, Dept Infect Dis, Chongqing, Peoples R China."

来源：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000888230300001

JCR分区：Q2

影响因子：5.7

年份：2022

卷号：12

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：children; pulmonary tuberculosis; extrapulmonary tuberculosis; CPA; molecular

摘要："The research of childhood tuberculosis is inadequate in china. The cross-priming amplification (CPA) of specific DNA in clinical samples is increasingly adopted for the diagnosis of childhood tuberculosis. In this study, a multicenter research was performed to investigate the incidence and characteristics of childhood tuberculosis in suspected populations mainly by CPA method. 851 children suspected of tuberculosis were enrolled in seven centers across China. All samples were tested by a CPA method and 159 subjects were tested by Xpert MTB/RIF and liquid culture method in parallel to assess the reliability of the CPA method. A positive result in any one of the three methods provided a definitive diagnosis of Mycobacterium tuberculosis complex (MTBC) infection. The MTBC-positive rate was 9.5% (81/851) by the combined methods; 93.8% of the cases were detected by CPA technology (76/81). The rate of pulmonary infection was significantly higher than that of extrapulmonary infection (7.1%, 60/851 vs 2.5%, 21/851; P < 0.001). Scrofula was the predominant type of extrapulmonary tuberculosis. The MTBC positive rates in 12-18-year-old group (middle school), was 28.4% (23/81), higher than in those under-six-year-old (preschool; 39/525) and the 6-41-year-old (primary school; 18/235) groups combined (P < 0.001). The MTBC positive rate in patients with a clear history of tuberculosis exposure was significantly higher than in cases in which there was no history of tuberculosis contact (35.3%, 18/51 vs 7.8%, 61/782; P < 0.001). In conclusion, this multicenter investigation showed that pulmonary tuberculosis and extrapulmonary tuberculosis are not uncommon in children in China, with teenagers being particularly susceptible to infection. The incidence of pulmonary tuberculosis in children is higher than that of extrapulmonary tuberculosis. History of exposure to tuberculosis is a high risk factor for childhood tuberculosis."

基金机构：Key Development Program of Children's Hospital of Fudan University Fund [EK2022ZX05]

基金资助正文："We thank all the technicians and hospitals participating in this investigation for their contributions. We thank Ustar Biotech (Hangzhou, China) for the CPA equipment and detection reagents provided for this project. Thanks to the Key Development Program of Children's Hospital of Fudan University (EK2022ZX05) Fund for supporting this study."