Footshock-Induced Activation of the Claustrum-Entorhinal Cortical Pathway in Freely Moving Mice

作者全名："Huang, Wushuang; Qin, Jing; Zhang, Chunqing; Qin, Han; Xie, Peng"

作者地址："[Huang, Wushuang; Qin, Jing; Xie, Peng] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurol, Chongqing 400016, Peoples R China; [Huang, Wushuang; Qin, Jing; Xie, Peng] Chongqing Med Univ, NHC Key Lab Diag & Treatment Brain Funct Dis, Chongqing 400016, Peoples R China; [Zhang, Chunqing] Army Med Univ, Xinqiao Hosp, Dept Neurosurg, Chongqing, Peoples R China; [Qin, Han] Chongqing Univ, Ctr Neurointelligence, Sch Med, Chongqing 400044, Peoples R China"

通信作者："Xie, P (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Neurol, Chongqing 400016, Peoples R China.; Xie, P (通讯作者)，Chongqing Med Univ, NHC Key Lab Diag & Treatment Brain Funct Dis, Chongqing 400016, Peoples R China.; Qin, H (通讯作者)，Chongqing Univ, Ctr Neurointelligence, Sch Med, Chongqing 400044, Peoples R China."

来源：PHYSIOLOGICAL RESEARCH

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000890049600004

JCR分区：Q4

影响因子：2.1

年份：2022

卷号：71

期号：5

开始页：695

结束页：701

文献类型：Article

关键词：Claustrum; Fear; Footshock; Fiber photometry; GCaMP

摘要："Footshock is frequently used as an unconditioned stimulus in fear conditioning behavior studies. The medial entorhinal cortex (MEC) contributes to fear learning and receives neuronal inputs from the claustrum. However, whether footshocks can induce a neuronal response in claustrum-MEC (CLA-MEC) projection remains unknown. Here, we combined fiber-based Ca2+ recordings with a retrograde AAV labeling method to investigate neuronal responses of MEC-projecting claustral neurons to footshock stimulation in freely moving mice. We achieved successful Ca2+ recordings in both anesthetized and freely exploring mice. We found that footshock stimulation reliably induced neuronal responses to MEC-projecting claustral neurons. Therefore, the footshock-induced response detected in the CLA-MEC projection suggests its potential role in fear processing."

基金机构：National Natural Science Foundation of China; Fundamental Research Funds for the Central Universities; [82171463]; [2022CDJXY-024]

基金资助正文：Acknowledgements The authors are grateful to Ms. Jia Lou for help in composing and editing the layout of the figures. This work was supported by grants from the National Natural Science Foundation of China (82171463) to CZ and Fundamental Research Funds for the Central Universities (2022CDJXY-024) to HQ.