Detection of carcinoembryonic antigens using a wavy gold-silver alloy nanoplate enhanced surface plasmon resonance imaging biosensor
作者全名："Wu, Jiangling; Zhang, Qiongyuan; Kang, Lina; Wu, Xiaotian; Li, Daikun; Wang, Yi; Huang, Yu; Xue, Jianjiang"
作者地址："[Wu, Jiangling; Zhang, Qiongyuan; Kang, Lina; Wu, Xiaotian; Li, Daikun; Xue, Jianjiang] Chongqing Med Univ, Univ Town Hosp, Med Sci Res Ctr, Dept Clin Lab, Chongqing 401331, Peoples R China; [Huang, Yu] Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China; [Wang, Yi] Chongqing Normal Univ, Coll Chem, Chongqing Key Lab Green Synth & Applicat, Chongqing 401331, Peoples R China"
通信作者："Xue, JJ (通讯作者)，Chongqing Med Univ, Univ Town Hosp, Med Sci Res Ctr, Dept Clin Lab, Chongqing 401331, Peoples R China.; Huang, Y (通讯作者)，Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China."
来源：ANALYTICAL METHODS
ESI学科分类：CHEMISTRY
WOS号：WOS:000890142400001
JCR分区：Q2
影响因子：3.1
年份：2022
卷号：14
期号：45
开始页：4713
结束页：4720
文献类型：Article
关键词：
摘要："Carcinoembryonic antigen (CEA) is regarded as a promising broad spectrum tumor biomarker for clinical diagnosis, progression, and prognosis. Surface plasmon resonance imaging (SPRi) was considered as one of the powerful tools for immunoassay with advantages of label-free, real-time detection with high-throughput. Herein, wavy gold-silver alloy nanoplates functionalized with anti-CEA antibodies providing high protein loading capacity and high mass are used as signal enhancers for CEA detection through SPRi sandwich assay. The present method exhibits a dynamic range for CEA determination from 0.1 to 312.5 ng mL(-1) and a detection limit of 0.55 ng mL(-1), well below normal physiological levels. This biosensing approach demonstrates the advantages of wavy gold-silver alloy nanoplates compared to conventional gold nanoparticles as a signal amplifier to enhance the SPRi signal, which is expected to become a new prospect for detection of cancer markers in biomedical research and clinical diagnosis."
基金机构：Science and Technology Research Program of the Chongqing Education Commission of China [KJQN202200431]; Natural Science Foundation of Chongqing [cstc2018jcyjAX0251]; Project of the Basic and Frontier Funds in the Yuzhong District of Chongqing [20200154]; Scientific Research Funding [2019JC05]; Talents Project of the University-Town Hospital of Chongqing Medical University; Beibei District Science and Technology Agency Supported Program for Science and Technology Talents & Independent Innovation [2021-6]
基金资助正文："This work was funded by Science and Technology Research Program of the Chongqing Education Commission of China (grant no. KJQN202200431), the Natural Science Foundation of Chongqing (grant no. cstc2018jcyjAX0251). a Project of the Basic and Frontier Funds in the Yuzhong District of Chongqing (grant no. 20200154), Scientific Research Funding (account number 2019JC05), Talents Project of the University-Town Hospital of Chongqing Medical University and the Beibei District Science and Technology Agency Supported Program for Science and Technology Talents & Independent Innovation (2021-6)."
