Stress-induced myelin damage in the hippocampal formation in a rat model of depression

作者全名："Huang, Chun-Xia; Xiao, Qian; Zhang, Lei; Gao, Yuan; Ma, Jing; Liang, Xin; Tang, Jing; Wang, San-Rong; Luo, Yan-Min; Chao, Feng-Lei; Xiu, Yun; Tang, Yong"

作者地址："[Huang, Chun-Xia; Luo, Yan-Min] Chongqing Med Univ, Fac Basic Med Sci, Dept Physiol, Chongqing, Peoples R China; [Huang, Chun-Xia; Xiao, Qian; Zhang, Lei; Tang, Jing; Luo, Yan-Min; Chao, Feng-Lei; Tang, Yong] Chongqing Med Univ, Fac Basic Med Sci, Lab Stem Cell & Tissue Engn, Chongqing, Peoples R China; [Xiao, Qian] Chongqing Med Univ, Fac Basic Med Sci, Dept Radioact Med, Chongqing, Peoples R China; [Zhang, Lei; Tang, Jing; Chao, Feng-Lei; Tang, Yong] Chongqing Med Univ, Fac Basic Med Sci, Dept Histol & Embryol, Chongqing, Peoples R China; [Gao, Yuan] Chongqing Med Univ, Affiliated Hosp 1, Dept Geriatr, Chongqing, Peoples R China; [Ma, Jing] Chongqing Med Univ, Fac Basic Med Sci, Dept Anat, Chongqing, Peoples R China; [Liang, Xin] Chongqing Med Univ, Fac Basic Med Sci, Dept Pathophysiol, Chongqing, Peoples R China; [Wang, San-Rong] Chongqing Med Univ, Affiliated Hosp 2, Dept Rehabil, Chongqing, Peoples R China; [Xiu, Yun] Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China"

通信作者："Tang, Y (通讯作者)，Chongqing Med Univ, Fac Basic Med Sci, Dept Histol & Embryol, Chongqing, Peoples R China."

来源：JOURNAL OF PSYCHIATRIC RESEARCH

ESI学科分类：PSYCHIATRY/PSYCHOLOGY

WOS号：WOS:000890256700018

JCR分区：Q1

影响因子：4.8

年份：2022

卷号：155

期号：　

开始页：401

结束页：409

文献类型：Article

关键词：Hippocampal formation; Demyelination; Depression; Transmission electron microscopy; Stereology

摘要："Background: According to previous studies, myelin damage may be involved in the occurrence of depression. However, to date, no study has quantitatively investigated the changes in myelinated fibers and myelin sheaths in the hippocampal formation (HF) and hippocampal subfields in the context of depression. Methods: Male Sprague-Dawley (SD) rats (aged 4-5 weeks) were evenly divided into the control group and chronic unpredictable stress (CUS) group. Behavioral tests were performed, and then changes in myelinated fibers and myelin ultrastructure in hippocampal subfields in depression model rats were investigated using modern stereological methods and transmission electron microscopy techniques. Results: After a four-week CUS protocol, CUS rats showed depressive-like and anxiety-like behaviors. The total length and total volume of myelinated fibers were reduced in the CA1 region and DG in the CUS group compared with the control group. The total volumes of myelin sheaths and axons in the CA1 region but not in the DG were significantly lower in the CUS group than in the control group. The decrease in the total length of myelinated nerve fibers in the CA1 region in CUS rats was mainly due to a decrease in the length of myelinated fibers with a myelin sheath thickness of 0.15 mu m-0.20 mu m. Limitations: The exact relationship between the degeneration of myelin sheaths and depression-like, anxiety-like behaviors needs to be further investigated. Conclusions: CUS induces depression- and anxiety-like behaviors, and the demyelination in the CA1 region induced by 4 weeks of CUS might be an important structural basis for these behaviors."

基金机构："Natural Science Foundation of China (NSFC) [82171522, 81871073, 81501156]; General Project of Chongqing Frontier and Applied Basic Research Project [cstc2015jcyjA10020]; Natural Science Foundation of Chongqing, China [cstc2020jcyj-msxmX0354]"

基金资助正文："This work was supported in part by the Natural Science Foundation of China (NSFC, 82171522, 81871073, 81501156); the General Project of Chongqing Frontier and Applied Basic Research Project (No. cstc2015jcyjA10020); and the Natural Science Foundation of Chongqing, China (cstc2020jcyj-msxmX0354)."