"Comprehensive analysis of necroptosis-related long noncoding RNA to predict prognosis, immune status, and immunotherapeutic response in clear cell renal cell carcinoma"
作者全名："Tang, Haibin; Chen, Hualin; Yuan, Heng; Jin, Xiaoxiang; Chen, Gang"
作者地址："[Tang, Haibin; Chen, Hualin; Yuan, Heng; Jin, Xiaoxiang; Chen, Gang] Chongqing Med Univ, Dept Urol, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China"
通信作者："Chen, G (通讯作者)，Chongqing Med Univ, Dept Urol, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China."
来源：TRANSLATIONAL CANCER RESEARCH
ESI学科分类：BIOLOGY & BIOCHEMISTRY
关键词：Clear cell renal cell carcinoma (ccRCC); necroptosis; long noncoding RNA signature; immune infiltration; The Cancer Genome Atlas (TCGA)
摘要："Background: Necroptosis has been found to be associated with tumorigenesis and tumor progression. However, the prognostic effect of long noncoding RNAs (lncRNAs) associated with necroptosis in clear cell renal cell carcinoma (ccRCC) is still unclear. Methods: Pearson correlation analysis was used to identify necroptosis-related genes and lncRNAs obtained from The Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma (TCGA-KIRC) dataset. Least absolute shrinkage and selection operator (LASSO) regression and Cox regression analyses were used to identify a novel necroptosis-associated lncRNAs signature that significantly correlated with survival of ccRCC. Next, single sample gene set enrichment analysis (ssGSEA) was employed to assess the extent of infiltration with immune cells. Analyses to predict the half-maximal inhibitory concentration (IC50) of patients in different risk groups were also conducted. Moreover, follow-up data of an immunotherapy cohort were used to test for differences in the immunotherapeutic efficiency between two risk groups. Finally, patients with ccRCC were divided into two groups based on 6 prognostic lncRNAs.Results: We developed a signature of necroptosis-related lncRNAs, which was verified as an independent prognostic factor that can predict prognosis up to 7 years. Patients with higher risk scores were shown to have higher immune suppressive cell infiltration levels and expression of immune checkpoint genes, which suggests that these patients were in a state of immunosuppression. Patients in the low-risk group were found to have an increased response to immunotherapy. A prognostic prediction nomogram was conducted to predict long-term survival of patients. Cluster A tumors were considered hot tumors, since they were correlated with higher levels of immune infiltration and were more sensitive to immunotherapy.Conclusions: A comprehensive bioinformatics analysis was conducted, which found that the necroptosisassociated lncRNA signature might be a potent prognostic factor for patients with ccRCC, which could contribute to improved prognosis of these patients."
基金机构：Senior Medical Talents Program of Chongqing for Early and Mid Career Researchers from the Chongqing Health Commission Program [2019GDRC017]; Senior Medical Talents Program of Chongqing for Early and Mid Career Researchers - Chongqing Health Commission [2022GDRC014]
基金资助正文："Y This work was supported by the Senior Medical Talents Program of Chongqing for Early and Mid Career Researchers from the Chongqing Health Commission Program (No. 2019GDRC017) , and the Senior Medical Talents Program of Chongqing for Early and Mid Career Researchers supported by Chongqing Health Commission (No. 2022GDRC014) ."