An Evaluation of Different Digestion Methods for the Quantitation of Inorganic Elements in Human Hair Using ICP-MS

作者全名："Liu, Yue; Yang, Yang; Xia, Yin-Yin; de Seymour, Jamie V.; Zhao, De-Zhang; Li, Yang-Mei; Zhang, Hua; Han, Ting-Li"

作者地址："[Liu, Yue; Han, Ting-Li] Chongqing Med Univ, Dept Obstet & Gynaecol, Affiliated Hosp 2, Chongqing, Peoples R China; [Liu, Yue; Xia, Yin-Yin] Chongqing Med Univ, Res Ctr Med & Social Dev, Innovat Ctr Social Risk Governance Hlth, Sch Publ Hlth & Management,Dept Occupat & Environm, Chongqing, Peoples R China; [Yang, Yang; Zhang, Hua] Chongqing Med Univ, Dept Obstet & Gynaecol, Affiliated Hosp 1, Chongqing, Peoples R China; [Yang, Yang; Zhang, Hua; Han, Ting-Li] Chongqing Med Univ, Mass Spectrometry Ctr Maternal Fetal Med, Chongqing, Peoples R China; [de Seymour, Jamie V.] Massey Univ, Coll Hlth, Auckland, New Zealand; [Zhao, De-Zhang] Chongqing Med Univ, Coll Pharm, Chongqing, Peoples R China; [Li, Yang-Mei] Chongqing Med Univ, Res Ctr Med & Social Dev, Innovat Ctr Social Risk Governance Hlth, Sch Publ Hlth & Management, Chongqing, Peoples R China"

通信作者："Han, TL (通讯作者)，Chongqing Med Univ, Dept Obstet & Gynaecol, Affiliated Hosp 2, Chongqing, Peoples R China.; Zhang, H (通讯作者)，Chongqing Med Univ, Dept Obstet & Gynaecol, Affiliated Hosp 1, Chongqing, Peoples R China.; Zhang, H; Han, TL (通讯作者)，Chongqing Med Univ, Mass Spectrometry Ctr Maternal Fetal Med, Chongqing, Peoples R China."

来源：JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:000896979200001

JCR分区：Q3

影响因子：2.6

年份：2022

卷号：2022

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："The inorganic elements have unique properties in biochemical processes in humans. An increasing number of pathologies have been associated with essential element ions, such as lead, mercury, and cadmium. Hair has become an attractive clinical specimen for studying the longitudinal exposure to elements from the external environment. Inductively coupled plasma-mass spectrometry (ICP-MS) coupled with nitric acid (HNO3) digestion is the most common approach for determining inorganic elements from human hair. This study aims to optimize the digestion method for the absolute quantitation of 52 elements using ICP-MS, for a large cohort study in human hair. Five different HNO3 (65%) digestion methods were investigated and evaluated for their internal standard solution stability, reproducibility, element coverage, and standard solution recovery efficiency, namely, room temperature for 24 h (RT), 90 degrees C for 4 h (T90), ultrasonic-assisted digestion (UltraS), programmed digestion of microwave digestion (MicroD), and ordinary microwave oven digestion (O-MicroD). Our results demonstrated that O-MicroD, MicroD, and RT were the best performing digestion methods for coefficient of variation (CV) scores, coverage, and recovery efficiency, respectively. In particular, the O-MicroD method detected multiple elements in a small quantity of hair (3 mg), with minimum nitric acid usage (200 mu l) and a short digestion time (30 min). The O-MicroD method had excellent reproducibility, as demonstrated by a continuous thousand injections of hair samples with three internal standards (CV: Rh-103 = 3.59%, In-115 = 3.61%, and Bi-209 = 6.31%). Future studies of the elemental content of hair should carefully select their digestion method to meet the primary purpose of their study."

基金机构："National Natural Science Foundation of China [81871185, 81971406]; 111 Project [(2016)32]; Kuanren Talents Programs of Second Affiliated Hospital of Chongqing Medical University; Chongqing Science and Technology Commission [cstc2021jcyj-msxmX0213]; Chongqing Municipal Education Commission [KJZD-K202100407]; Chongqing Health Commission and Chongqing Science and Technology Commission [2021MSXM121, 2020MSXM101]; Smart Medicine Research Project of Chongqing Medical University [ZHYX202103]; Joint Health Research Council New Zealand-National Science Foundation of China Biomedical Research Fund [19/804]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (No. 81871185, 81971406), The 111 Project (Yuwaizhuan (2016)32), Kuanren Talents Programs of Second Affiliated Hospital of Chongqing Medical University, Chongqing Science and Technology Commission (cstc2021jcyj-msxmX0213), Chongqing Municipal Education Commission (KJZD-K202100407), Chongqing Health Commission and Chongqing Science and Technology Commission (2021MSXM121, 2020MSXM101), Smart Medicine Research Project of Chongqing Medical University (No. ZHYX202103), and a Joint Health Research Council New Zealand-National Science Foundation of China Biomedical Research Fund (19/804)."