Activating Wnt/beta-Catenin Signaling in Osteocytes Promotes Osteogenic Differentiation of BMSCs through BMP-7
作者全名："Zhang, Yining; Zhao, Yixin; Xie, Zhengsong; Li, Molin; Liu, Yujiao; Tu, Xiaolin"
作者地址："[Zhang, Yining; Zhao, Yixin; Xie, Zhengsong; Li, Molin; Liu, Yujiao; Tu, Xiaolin] Chongqing Med Univ, Inst Life Sci, Lab Skeletal Dev & Regenerat, Chongqing 400016, Peoples R China"
通信作者："Zhang, YN; Tu, XL (通讯作者)，Chongqing Med Univ, Inst Life Sci, Lab Skeletal Dev & Regenerat, Chongqing 400016, Peoples R China."
来源：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
关键词：osteocytes; stem cells; bone formation; Wnt signaling; bone morphogenetic protein-7
摘要："Bone formation is critically needed in orthopedic clinical practice. We found that, bone morphogenetic protein-7 (BMP-7) gene expression was significantly increased in fractured mice, which activates canonical Wnt signaling exclusively in osteocytes. Wnt and BMP signaling appear to exhibit synergistic or antagonistic effects in different kinds of cells. However, the communication between Wnt/beta-catenin signaling and BMP signaling in osteocytes is almost unknown. Our study verified in vitro that BMP-7 expression was significantly increased when Wnt signaling was activated in osteocytes. Next, BMP-7 in osteocytes was overexpressed using an adenovirus, the osteogenesis of bone marrow stem cells (BMSCs) was enhanced, when cocultured with osteocytes. On the contrary, BMP-7 in osteocytes was silenced using an adenovirus, the osteogenesis of bone marrow stem cells (BMSCs) was weakened. In addition, the osteogenesis of BMSCs was no longer promoted by Wnt-activated osteocytes when BMP-7 was silenced. Therefore, the results showed that BMP-7 mediated the anabolic actions of Wnt/beta-catenin signaling in osteocytes. Our study provides new evidence for the clinical application of BMP-7-overexpressed osteocytes."
基金机构：National Natural Science Foundation of China; Scientific Research Project of Zhejiang Provincial Department of Health; Chongqing Graduate Scientific Research Innovation Project; ; [2023KY071]; [CYB19167]
基金资助正文："This work is supported by grants from the National Natural Science Foundation of China (82072450), Scientific Research Project of Zhejiang Provincial Department of Health (2023KY071), Chongqing Graduate Scientific Research Innovation Project (CYB19167)."