EIF4G1 Is a Potential Prognostic Biomarker of Breast Cancer

作者全名："Li, Kun; Tan, Guangqing; Zhang, Xin; Lu, Weiyu; Ren, Jingyi; Si, Yuewen; Adu-Gyamfi, Enoch Appiah; Li, Fangfang; Wang, Yingxiong; Xie, Biao; Wang, Meijiao"

作者地址："[Li, Kun; Tan, Guangqing; Zhang, Xin; Lu, Weiyu; Ren, Jingyi; Si, Yuewen; Wang, Meijiao] Chongqing Med Univ, Coll Basic Med, Dept Physiol, Chongqing 400016, Peoples R China; [Adu-Gyamfi, Enoch Appiah] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA; [Li, Fangfang; Wang, Yingxiong; Wang, Meijiao] Chongqing Med Univ, Sch Publ Hlth & Management, Joint Int Res Lab Reprod Dev, Minist Educ China, Chongqing 400016, Peoples R China; [Xie, Biao] Chongqing Med Univ, Sch Publ Hlth & Management, Dept Biostat, Chongqing 400016, Peoples R China"

通信作者："Wang, MJ (通讯作者)，Chongqing Med Univ, Coll Basic Med, Dept Physiol, Chongqing 400016, Peoples R China.; Wang, MJ (通讯作者)，Chongqing Med Univ, Sch Publ Hlth & Management, Joint Int Res Lab Reprod Dev, Minist Educ China, Chongqing 400016, Peoples R China.; Xie, B (通讯作者)，Chongqing Med Univ, Sch Publ Hlth & Management, Dept Biostat, Chongqing 400016, Peoples R China."

来源：BIOMOLECULES

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000902285500001

JCR分区：Q2

影响因子：5.5

年份：2022

卷号：12

期号：12

开始页：　

结束页：　

文献类型：Article

关键词：breast cancer; prognosis; immune infiltration; immunohistochemistry

摘要："Background: Breast cancer (BRCA) is one of the most common cancers in women worldwide and a leading cause of death from malignancy. This study was designed to identify a novel biomarker for prognosticating the survival of BRCA patients. Methods: The prognostic potential of eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) was assessed using RNA sequencing (RNA-seq) data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) as training cohort and validation set, respectively. The functional enrichment analysis of differentially expressed genes (DEGs) was performed. The relationship between EIF4G1 and tumor microenvironment (TME) was analyzed. Immunotherapy responses were explored by the immunophenoscores (IPS) and tumor immune dysfunction and exclusion (TIDE) score. The Connectivity Map (CMap) was used to discover potentially effective therapeutic molecules against BRCA. Immunohistochemistry (IHC) was applied to compare the protein levels of EIF4G1 in normal and cancer tissues and to verify the prognostic value of EIF4G1. Results: BRCA patients with increased expression of EIF4G1 had a shorter overall survival (OS) in all cohorts and results from IHC. EIF4G1-related genes were mainly involved in DNA replication, BRCA metastasis, and the MAPK signaling pathway. Infiltration levels of CD4(+)-activated memory T cells, macrophages M0, macrophages M1, and neutrophils were higher in the EIF4G1 high-expression group than those in the EIF4G1 low-expression group. EIF4G1 was positively correlated with T cell exhaustion. Lower IPS was revealed in high EIF4G1 expression patients. Five potential groups of drugs against BRCA were identified. Conclusion: EIF4G1 might regulate the TME and affect BRCA metastasis, and it is a potential prognostic biomarker and therapeutic target for BRCA."

基金机构："National Natural Science Foundation of China; Chongqing Natural Science Foundation [82171624, 82204159]; Science and Technology Project of Chongqing Yuzhong District [cstc2020jcyj-msxmX0294]; Scientific Research & Innovation Experiment Project of Chongqing Medical University [20200103]; Chongqing Postdoctoral Science Foundation [SRIEP202002, SRIEP202106]; [cstc2021jcyj-bsh0029]"

基金资助正文："This study was supported by grants from the National Natural Science Foundation of China (Nos. 82171624, 82204159), the Chongqing Natural Science Foundation (No. cstc2020jcyj-msxmX0294), the Science and Technology Project of Chongqing Yuzhong District (No. 20200103), the Scientific Research & Innovation Experiment Project of Chongqing Medical University (SRIEP202002, SRIEP202106), and the Chongqing Postdoctoral Science Foundation (No. cstc2021jcyj-bsh0029)."