IKZF3 modulates cerebral ischemia/reperfusion injury by inhibiting neuroinflammation
作者全名："Meng, Changchang; Chen, Shiyu; He, Qi; Tan, Junyi; Wu, Jingxian; Zhao, Jing"
作者地址："[Meng, Changchang; He, Qi; Tan, Junyi; Zhao, Jing] Chongqing Med Univ, Dept Pathophysiol, Chongqing 400016, Peoples R China; [Chen, Shiyu; Wu, Jingxian] Chongqing Med Univ, Dept Pathol, Chongqing 400016, Peoples R China; [Wu, Jingxian; Zhao, Jing] Chongqing Med Univ, Inst Neurosci, Chongqing 400016, Peoples R China"
通信作者："Wu, JX; Zhao, J (通讯作者)，Chongqing Med Univ, Inst Neurosci, Chongqing 400016, Peoples R China."
ESI学科分类：PHARMACOLOGY & TOXICOLOGY
关键词：Neuroinflammation; Inflammatory factors; Cerebral ischemia; reperfusion injury; IKZF3; NF-?B
摘要："Neuroinflammation is a key mediator to the pathogenic cascades induced by cerebral ischemia-reperfusion (I/R) injury. IKZF3, a key zinc finger transcription factor in the Ikaros family, has already been shown to modulate a wide range of cell functions and the production of inflammatory mediators. However, the effects of IKZF3 on inflammation and the potential mechanism after cerebral I/R injury remain unclear. In this study, we evaluated the effect of IKZF3 on HT-22 cells under oxygen-glucose deprivation and reoxygenation (OGD/R) in vitro and in mice with MACO in vivo. We found that IKZF3 expression peaked at 12 h after MCAO and OGD/R, and there was high expression of IKZF3 in brain tissues and HT-22 cells. IKZF3 knockdown exacerbated the damage by OGDinduced HT-22 cells injury and MCAO-induced brain injury in mice by regulating the production of inflammatory factors, which promoted the phosphorylation and nuclear transfer of NF-kappa B and may bind with NF-kappa B-p65 in vivo and in vitro. Our results suggested that IKZF3 may provide a new target in improve neurological recovery and reducing neuroinflammation after cerebral I/R injury."
基金机构："Natural Science Foundation of Chongqing Science and Technology Committee, China; National Natural Science Foundation of China; Top-notch Postgraduate Talent Cultivation program of Chongqing Medical University; Graduate Scientific Research Innovation Project of Chongqing municipality; Chongqing Talent Program of Contract Program; [CSTC2021jcyj- msxmX0001]; ; [BJRC202103]; [CSTC2022ycjh-bgzxm0057]"
基金资助正文："This work was supported by the Natural Science Foundation of Chongqing Science and Technology Committee, China (CSTC2021jcyj- msxmX0001 to JZ) and the National Natural Science Foundation of China (grant number: 82071305 to JZ) , and the Top-notch Postgraduate Talent Cultivation program of Chongqing Medical University (grant number: BJRC202103 to CCM) , and Graduate Scientific Research Innovation Project of Chongqing municipality (grant num- ber:2021cys21214 to SYC) , and the Chongqing Talent Program of Contract Program (grant number:CSTC2022ycjh-bgzxm0057 to JXW) ."