DLP-based bioprinting of void-forming hydrogels for enhanced stem-cell-mediated bone regeneration

作者全名："Tao, Jie; Zhu, Shunyao; Liao, Xueyuan; Wang, Yu; Zhou, Nazi; Li, Zhan; Wan, Haoyuan; Tang, Yaping; Yang, Sen; Du, Ting; Yang, Yang; Song, Jinlin; Liu, Rui"

作者地址："[Tao, Jie; Zhu, Shunyao; Liao, Xueyuan; Wang, Yu; Zhou, Nazi; Wan, Haoyuan; Tang, Yaping; Yang, Sen; Yang, Yang; Liu, Rui] Third Mil Med Univ, Army Med Univ, Daping Hosp, Dept Stomatol, Chongqing 400042, Peoples R China; [Li, Zhan] Army Med Univ, Daping Hosp, Dept Stem Cell & Regenerat Med, State Key Lab Trauma Burn & Combined Injury, Chongqing 400042, Peoples R China; [Song, Jinlin] Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China; [Du, Ting] Chengdu Med Coll, Noncoding RNA & Drug Discovery Key Lab Sichuan Pr, Chengdu, Sichuan, Peoples R China"

通信作者："Liu, R (通讯作者)，Third Mil Med Univ, Army Med Univ, Daping Hosp, Dept Stomatol, Chongqing 400042, Peoples R China.; Song, JL (通讯作者)，Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China."

来源：MATERIALS TODAY BIO

ESI学科分类：　

WOS号：WOS:000908044500004

JCR分区：Q1

影响因子：8.2

年份：2022

卷号：17

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：3D bioprinting; Void-forming; Macroporous hydrogel; Bone marrow stem cells; Bone regeneration

摘要："The integration of 3D bioprinting and stem cells is of great promise in facilitating the reconstruction of cranial defects. However, the effectiveness of the scaffolds has been hampered by the limited cell behavior and functions. Herein, a therapeutic cell-laden hydrogel for bone regeneration is therefore developed through the design of a void-forming hydrogel. This hydrogel is prepared by digital light processing (DLP)-based bioprinting of the bone marrow stem cells (BMSCs) mixed with gelatin methacrylate (GelMA)/dextran emulsion. The 3D-bioprinted hydrogel can not only promote the proliferation, migration, and spreading of the encapsulated BMSCs, but also stimulate the YAP signal pathway, thus leading to the enhanced osteogenic differentiation of BMSCs. In addition, the in vivo therapeutic assessments reveal that the void-forming hydrogel shows great potential for BMSCs delivery and can significantly promote bone regeneration. These findings suggest that the unique 3D-bioprinted void-forming hydrogels are promising candidates for applications in bone regeneration."

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