Neuroblasts migration under control of reactive astrocyte-derived BDNF: a promising therapy in late neurogenesis after traumatic brain injury

作者全名:"Wu, Na; Sun, Xiaochuan; Zhou, Chao; Yan, Jin; Cheng, Chongjie"

作者地址:"[Wu, Na; Sun, Xiaochuan; Zhou, Chao; Yan, Jin; Cheng, Chongjie] Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400010, Peoples R China; [Wu, Na] Chongqing Univ, Dept Pediat Surg, Three Gorges Hosp, Chongqing, Peoples R China"

通信作者:"Cheng, CJ (通讯作者),Chongqing Med Univ, Dept Neurosurg, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400010, Peoples R China."












关键词:Traumatic brain injury; Neuroblast; Neuronal migration; Brain-derived neurotrophic factor; CC chemokine ligand 2; Monocyte chemoattractant protein-1

摘要:"Background: Traumatic brain injury (TBI) is a disease with high mortality and morbidity, which leads to severe neurological dysfunction. Neurogenesis has provided therapeutic options for treating TBI. Brain derived neurotrophic factor (BDNF) plays a key role in neuroblasts migration. We aimed to investigate to the key regulating principle of BDNF in endogenous neuroblasts migration in a mouse TBI model. Methods: In this study, controlled cortical impact (CCI) mice (C57BL/6J) model was established to mimic TBI. The sham mice served as control. Immunofluorescence staining and enzyme-linked immunosorbent assay were performed on the CCI groups (day 1, 3, 7, 14 and 21 after CCI) and the sham group. All the data were analyzed with Student's t-test or one-way or two-way analysis of variance followed by Tukey's post hoc test. Results: Our results revealed that neuroblasts migration initiated as early as day 1, peaking at day 7, and persisted till day 21. The spatiotemporal profile of BDNF expression was similar to that of neuroblasts migration, and BDNF level following CCI was consistently higher in injured cortex than in subventricular zone (SVZ). Reactive astrocytes account for the major resource of BDNF along the migrating path, localized with neuroblasts in proximity. Moreover, injection of exogenous CC chemokine ligand 2 (CCL2), also known as monocyte chemoattractant protein-1, at random sites promoted neuroblasts migration and astrocytic BDNF expression in both normal and CCI mice (day 28). These provoked neuroblasts can also differentiate into mature neurons. CC chemokine ligand receptor 2 antagonist can restrain the neuroblasts migration after TBI. Conclusions: Neuroblasts migrated along the activated astrocytic tunnel, directed by BDNF gradient between SVZ and injured cortex after TBI. CCL2 might be a key regulator in the above endogenous neuroblasts migration. Moreover, delayed CCL2 administration may provide a promising therapeutic strategy for late neurogenesis post-trauma."

基金机构:National Natural Science Foundation of China; National Natu-ral Science Foundation of China [82071397]; [82172193]

基金资助正文:"This work was supported by the National Natural Science Foundation of China, No. 82071397 (to X.C.S.); this work was supported by the National Natu-ral Science Foundation of China, No. 82172193 (to C.J.C). The funding sources had no role in study conception and design, data analysis or interpretation, paper writing or deciding to submit this paper for publication."