Preparation and characterization of a gemini surfactant-based biomimetic complex for gene delivery
作者全名："Lu, Zhou; Zongjie, Gan; Qianyu, Zhang; Xueyan, Liu; Kexin, Wu; Baoyan, Chen; Ran, Tao; Fang, Ren; Hui, Hu; Huali, Chen"
作者地址："[Lu, Zhou; Zongjie, Gan; Qianyu, Zhang; Xueyan, Liu; Kexin, Wu; Baoyan, Chen; Ran, Tao; Fang, Ren; Hui, Hu; Huali, Chen] Chongqing Med Univ, Coll Pharm, Chongqing Key Lab Biochem & Mol Pharmacol, Med Sch Rd, Chongqing 400042, Peoples R China"
通信作者："Huali, C (通讯作者)，Chongqing Med Univ, Coll Pharm, Chongqing Key Lab Biochem & Mol Pharmacol, Med Sch Rd, Chongqing 400042, Peoples R China."
来源：EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
ESI学科分类：PHARMACOLOGY & TOXICOLOGY
关键词：Gemini surfactant; Red blood cell membrane; Helper lipid; Transfection efficiency; Gene delivery
摘要："Gemini surfactants (GS) have been explored as non-viral gene delivery systems. Nevertheless, their cytotoxicity and the limitations in the in vivo studies have impeded their development. To attenuate toxicity and further explore their possibilities in gene delivery, a series of GS (18-7-18)-based gene delivery systems complexed with red blood cell membranes (RBCM) or/and DOPE-PEG2000 (DP) were prepared and evaluated. EGFP-encoding plasmids were delivered via GS-based complexes and the efficiency of gene transfection was evaluated by im-aging of the major organs after intravenous administration in mice and qPCR quantification in hepatocytes. In order to assess the safety of GS-based complexes, the hemolysis test, serum biochemical indices, H&E staining and CCK-8 test were examined. The results revealed that EGFP was primarily expressed in livers, and all com-plexes showed minimal acute toxicity to major organs. Moreover, we found that the dual incorporation of RBCM and DP could significantly elevate the transfection efficiency and cell viability in hepatocytes. Overall, the results indicated that GS-based complexes possessed great potential as vectors for gene delivery both in vivo and in vitro and the dual incorporation of RBCM and DP could be a promising gene delivery approach with high transfection efficacy and low toxicity."
基金机构：Chongqing Basic and Frontier Research Project; Science and Technology Research Program of Chongqing Municipal Education Commission; [CSTC2018jcyjAX0126]; [KJQN202000407]; [KJQN202200451]
基金资助正文："Funding This work was supported by the Chongqing Basic and Frontier Research Project (grant No. CSTC2018jcyjAX0126) , the Science and Technology Research Program of Chongqing Municipal Education Commission (grant No. KJQN202000407 and No. KJQN202200451)"