Changes in type 2 innate lymphoid cells and serum cytokines in sublingual immunotherapy in pediatric patients with allergic rhinitis

作者全名："Wang, Xiaoqiang; Shen, Yang; Hong, Suling; Kang, Houyong; Ke, Xia"

作者地址："[Wang, Xiaoqiang; Shen, Yang; Hong, Suling; Kang, Houyong; Ke, Xia] Chongqing Med Univ, Dept Otolaryngol Head & Neck Surg, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China"

通信作者："Ke, X (通讯作者)，Chongqing Med Univ, Dept Otolaryngol Head & Neck Surg, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：BMC PEDIATRICS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000911486100001

JCR分区：Q2

影响因子：2.4

年份：2023

卷号：23

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Sublingual immunotherapy; Allergic rhinitis; Type 2 innate lymphocytes; Pediatric patients

摘要："Background Type 2 innate lymphoid cells (ILC2) are upregulated in childhood allergic rhinitis (AR) and are associated with AR severity. This study aimed to investigate changes in the ILC2 milieu in pediatric patients with AR after sublingual immunotherapy (SLIT).Methods Forty- pediatric patients with AR received house dust mite (HDM) allergen extract for SLIT group and thirty pediatric patients received placebo in the study, respectively. The levels of ILC2, ILC2-related cytokines (IL-5/IL-13) and their transcription factors (GATA binding protein 3, retinoic acid-related orphan receptor alpha) in the circulation were assessed after 1- and 2-year SLIT. Moreover, peripheral blood mononuclear cells (PBMCs) in patients were prepared and stimulated by recombinant thymic stromal lymphopoietin, IL-25, and IL-33 after 2-year SLIT. Subsequently, the levels of ILC2, IL-5, and IL-13 were tested.Results The frequency of ILC2 and the levels of their transcription factors in the circulation were significantly decreased after SLIT in the SLIT group. The levels of ILC2-related cytokines in the SLIT group showed the same trend. The frequency of ILC2 was positively correlated with transcription factors and cytokines after SLIT. SLIT was observed to reduce the ability of HDM sensitization to generate the ILC2 milieu in PBMCs.Conclusions Changes in the ILC2 milieu may be correlated with the curative effect and immune regulation function of SLIT. Our results suggested that the regulatory effect on ILC2 is part of the therapeutic mechanism of SLIT."

基金机构：Chongqing Natural Science Foundation; [cstc2019jcyj-msxmX0862]; [cstc2019jcyj-msxmX0506]

基金资助正文："This work was supported by the Chongqing Natural Science Foundation [grant number: cstc2019jcyj-msxmX0862, cstc2019jcyj-msxmX0506].The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data; and in the decision to submit the article for publication."