Association between IL-38 and inflammatory indicators in patients with bacterial pneumonia

作者全名："Sun, Xiaoyu; Zhou, Jie; Huang, Wenjuan; Wang, Bo; Zhang, Yi; Duan, Liang; Chen, Weixian"

作者地址："[Sun, Xiaoyu; Wang, Bo; Zhang, Yi; Duan, Liang; Chen, Weixian] Chongqing Med Univ, Dept Lab Med, Affiliated Hosp 2, 74 Linjiang Rd, Chongqing, Peoples R China; [Zhou, Jie] Chongqing Med Univ, Dept Clin Lab, Childrens Hosp, Chongqing, Peoples R China; [Zhou, Jie] Chongqing Med Univ, Key Lab Child Dev & Disorders, Minist Educ, Childrens Hosp, Chongqing, Peoples R China; [Zhou, Jie] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Childrens Hosp, Chongqing, Peoples R China; [Zhou, Jie] Chongqing Med Univ, China Int Sci & Technol Cooperat Base Child Dev &, Childrens Hosp, Chongqing, Peoples R China; [Zhou, Jie] Chongqing Key Lab Pediat, Chongqing, Peoples R China; [Huang, Wenjuan] Chongqing Orthoped Hosp Tradit Chinese Med, Chongqing 400010, Peoples R China"

通信作者："Duan, L; Chen, WX (通讯作者)，Chongqing Med Univ, Dept Lab Med, Affiliated Hosp 2, 74 Linjiang Rd, Chongqing, Peoples R China."

来源：CYTOKINE

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000913251000001

JCR分区：Q3

影响因子：3.8

年份：2023

卷号：161

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Interleukin-38; Anti-inflammation; Bacterial pneumonia; Biomarker

摘要："Background: IL-38, a recently discovered cytokine of IL-1 family, exerts immunoregulatory activities in multi-type inflammatory diseases. However, its expression level and underlying clinical importance for IL-38 in respiratory bacterial infections remain unknown.Methods: Thirty-five patients with bacterial pneumonia and twenty age-and gender-matched healthy individuals were enrolled in the study to determine serum IL-38 concentrations by ELISA. Then, the correlation between serum IL-38 levels and clinical features were analyzed and ROC curve was used to evaluate the potential diagnostic value for bacterial infections. In vitro, LPS-stimulated human respiratory epithelial cell model was employed to explore immunomodulatory mechanism of IL-38 in pulmonary infections. Results: Elevated serum levels of IL-38 were determined in patients with bacterial pneumonia when compared with healthy controls. In addition, serum IL-38 levels were negatively correlated with clinical inflammation parameters, including WBC count, CRP, PCT and proinflammatory IL-6 and IL-8. In vitro, we demonstrated that recombinant IL-38 was able to remarkably inhibit expression of proinflammatory IL-6, IL-8, IL-1 beta and TNF-alpha as well as adhesion molecule ICAM-1, which were partially mediated by attenuated activation of STAT3 and NF-Kappa B signal cascades in BEAS-2B cells. Furthermore, we identified the diagnostic efficiency of IL-38 in discriminating patients with bacterial pneumonia from healthy individuals.Conclusions: Our study indicates higher serum IL-38 levels in patients with bacterial pneumonia are involved in anti-inflammatory activities in respiratory infections revealing a critical role of IL-38 in attenuating excessive pulmonary inflammation against exogenous pathogens. More importantly, IL-38 exhibited a potential novel biomarker for bacterial pneumonia. Thus, our data may provide useful insights for both clinical and basic research for bacterial pneumonia diagnosis."

基金机构：Kuanren Talents Program of the second affiliated hospital of Chongqing medical university; Ministry of Ed-ucation Key Laboratory of Child Development and Disorders General Basic Research Project; [GBRP-202118]

基金资助正文：Acknowledgments This work was supported by Kuanren Talents Program of the second affiliated hospital of Chongqing medical university and Ministry of Ed-ucation Key Laboratory of Child Development and Disorders General Basic Research Project (GBRP-202118) .