Synergistic effect of bovine cateslytin-loaded nanoparticles combined with ultrasound against Candida albicans biofilm

作者全名："Liu, Xian; Xu, Shan; Chen, Xiaoying; Kang, Shengnan; Liu, Jinglun; Jiang, Qihua; Zhang, Shu; Hao, Lan; Ran, Haitao; Huang, Wenxiang; Zhang, Dan"

作者地址："[Liu, Xian; Chen, Xiaoying; Kang, Shengnan; Liu, Jinglun; Zhang, Dan] Chongqing Med Univ, Affiliated Hosp 1, Dept Emergency, Chongqing, Peoples R China; [Liu, Xian; Chen, Xiaoying; Kang, Shengnan; Liu, Jinglun; Zhang, Dan] Chongqing Med Univ, Affiliated Hosp 1, Crit Care Unit, Chongqing, Peoples R China; [Xu, Shan] Chongqing Med Univ, Affiliated Hosp 2, Dept Emergency, Chongqing, Peoples R China; [Jiang, Qihua] Chongqing Med Univ, Coll Pharm, Chongqing, Peoples R China; [Zhang, Shu] Chongqing Med Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Chongqing, Peoples R China; [Hao, Lan; Ran, Haitao] Chongqing Med Univ, Affiliated Hosp 2, Chongqing Key Lab Ultrason Mol Imaging, Chongqing, Peoples R China; [Huang, Wenxiang] Chongqing Med Univ, Affiliated Hosp 1, Infect Dis Dept, Key Lab Infect & Parasit Dis Chongqing, Chongqing, Peoples R China"

通信作者："Zhang, D (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Emergency, Chongqing, Peoples R China.; Zhang, D (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Crit Care Unit, Chongqing, Peoples R China."

来源：FUTURE MEDICINAL CHEMISTRY

ESI学科分类：CHEMISTRY

WOS号：WOS:000913832000001

JCR分区：Q3

影响因子：3.2

年份：2023

卷号：15

期号：1

开始页：43

结束页：55

文献类型：Article

关键词：antibacterial polypeptide; biofilm; Candida albicans; cateslytin; low-frequency ultrasound; nanoparticles

摘要："Purpose: To investigate the synergistic effect of bovine cateslytin-loaded nanoparticles (bCAT-NPs) combined with ultrasound against Candida albicans biofilm and uncover the underlying mechanism. Methods: bCAT-NPs were prepared by the double emulsion method, and toxicity was observed by the hemolysis ratio. The metabolic activity and viable cell biomass, morphology and membrane permeability of C. albicans biofilm were observed. The expression of ALS3 mRNA, the content of reactive oxygen species, was detected. Finally, bCAT structure was analyzed. Results & conclusion: The hemolysis ratio of the bCAT-NPs group was significantly lower than that of the bCAT group. bCAT-NPs combined with ultrasound significantly reduced biofilm metabolic activity, inhibited the formation of hyphae, decreased the expression of ALS3 mRNA and increased the intracellular reactive oxygen species content. In the in vivo experiments, the colony-forming units/ml in the ultrasound+bCAT-NPs group decreased, and a few planktonic fungal cells were observed."

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